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Part no: 9548
1
Mounting on to a Microscope
The microscope make and model was specified at
the time of order, and the clamping set or adaptor
plate are supplied specific to that set up.
Note: The CSS450 stage requires the objective
lenses to have a minimum working distance of
7.4mm.
Mounting on to a Dovetail
The following description for mounting the
stage on the Linkam Imaging Station can
also be applied to microscopes which have
similar circular dovetail substage assemblies.
Attach the Curved Clamps (part no. 9548) to the
Microscope Fixing Plate (1), using the supplied
hex screws (2.5mm) and to the outer most holes
of the Microscope Fixing Plate.
The two curved clamps are used to fix the
CSS450 to the microscope. To do this; adjust the
two positioning screws (2) and thumb screw (3)
so that they are showing about 5mm of thread on
the inside edge of the Curved Clamps. This will
roughly position the stage in the centre of the
dovetail.
Place the Base onto the dovetail and using a 5x or
10X objective lens, focus on the aperture of the
heating block. Using a hex screw driver to adjust
the two positioning hex screws (2), so that the
aperture is in the centre field of view of the opti-
cal light path. Then use the thumb screw (3) to
lock the stage in place.
Set up Kohler Illumination
Kohler illumination is a technique that provides
optimum resolution and contrast in a light micro-
scope by aligning and focussing the transmitted
illumination and critically setting the apertures of
the microscope to best match the objective lens
Numerical Aperture.
To use Kohler illumination with the CSS450, a
condenser lens with a minimum working distance
of 15mm is needed. Contact Linkam for further
information about condenser extension lenses.
For information about setting up Kohler Illumina-
tion and how to set up your microscope. See the
extremely informative ‘microscopy Primer’ on the
Molecular Expressions website.
http://micro.magnet.fsu.edu/primer/index.html