Luminex Amnis FlowSight

Questions and Answers

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nenglishNov 22, 2025
How to fix unstable fluidics (air or clog in system) on Luminex Amnis FlowSight?
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  Michael ShepherdNov 22, 2025
  To resolve unstable fluidics (air or clog in system) in Luminex Measuring Instruments, first, ensure a sufficient sample volume is used; 50 ?l is recommended. To clear any air bubbles, run the purge bubbles script. If detergents or foaming agents are causing bubbles, remove them and re-suspend the sample in dPBS, then run the purge bubbles script. For air bubbles in fluid lines, run the sterilize script, followed by the startup script, then load calibration beads to verify the system runs normally. If there's a clog in the fluid lines, filter the sample with a 70 ?m nylon cell strainer, then run the sterilize and startup scripts, and verify with calibration beads. If the sample is too concentrated, dilute it to 1x10^7 cells/mL, strain it through a 70 ?m nylon mesh, and run the purge bubble...
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Guy Lozano DVMAug 25, 2025
What to do if INSPIRE™ appears to freeze on Luminex Amnis FlowSight?
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  Jacob ReevesAug 26, 2025
  If INSPIRE™ appears to freeze on Luminex Measuring Instruments, first, check if the camera is running by clicking Run/Setup. If the camera is already running, try clicking Stop and then Run/Setup again. If imaging is paused, click Resume. If there are no objects in the current image view mode, select the all population in the cell view area. Lastly, if a script is running, wait until it completes, or click Abort Script to prematurely stop the operation.
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Michael RichardsonAug 27, 2025
What to do if the data file fails to collect on Luminex Amnis FlowSight Measuring Instruments?
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  tina29Aug 27, 2025
  If data file fails to collect on Luminex Measuring Instruments, it could be due to several reasons. First, ensure that events qualify for the region by viewing the region in the image gallery and updating the acquisition collection population appropriately. If the computer hard drive is full, back up and delete data to free up disk space. Verify the data destination folder is accessible using the browse button in the Acquisition Settings section if the file directory was lost. Finally, check Generate .rif and/or .fcs file in the file drop-down menu if no .rif or .fcs file was created.
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patricia47Aug 27, 2025
Why do the fluidics respond sluggishly on Luminex Amnis FlowSight Measuring Instruments?
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  Lisa PerezAug 28, 2025
  If the fluidics respond sluggishly on Luminex Measuring Instruments, it might be because the air buffer in the sheath syringe is incorrect. The sheath syringe should contain 2-4 mL of air to buffer the movement of the pump’s microstepper motor. If too little air is present, run the “start-up” script. Another possible cause is leaking fluid lines. In this case, look for leaking sheath with the system powered down and verify that the fluid lines mount snugly into position. If the issue persists, contact Luminex Technical Support.
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Lisa HarrisAug 29, 2025
How to troubleshoot objects appearing out of focus on Luminex Measuring Instruments?
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  Paul TorresAug 29, 2025
  To resolve the issue of objects appearing out of focus on Luminex Measuring Instruments, first, re-run the focus adjuster and frame offset calibration in ASSIST and verify that it passes. This addresses a potential issue with an incorrect camera line rate. If the problem persists, it could be due to excessive core stream variation caused by air or a clog in the fluidics. In this case, run the purge bubbles script from the instrument drop-down menu.
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Alexa WalkerSep 23, 2025
Why is scatter too dim on Luminex Amnis FlowSight?
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  Connie GlennSep 23, 2025
  If scatter is too dim or bright or changes over time on Luminex Measuring Instruments, it could be due to instrument experiencing large temperature variation. Allow the instrument to warm up by running for 15 minutes. Another cause could be laser power set too high or low, increase or decrease the 785 SSC laser power.
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Allison LeeSep 16, 2025
Why is fluorescence too bright on Luminex Amnis FlowSight?
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  Miss Linda McdonaldSep 17, 2025
  If fluorescence is too bright, images have a contrasting color, or appear flat on Luminex Measuring Instruments, the image display settings are likely set too high. To correct this, decrease the image display gain and change to linear in the appropriate camera channel.
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Miss Lindsey Norman MDSep 18, 2025
Why do images appear pixelated on Luminex Measuring Instruments?
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  Kimberly RobertsSep 18, 2025
  If images appear pixelated or larger than normal on Luminex Measuring Instruments, it's likely because the image gallery zoom is active. Use the – magnifying glass to zoom out and restore the native image size.
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Tammie JordanSep 8, 2025
What to do if INSPIRE™ fails to launch on Luminex Amnis FlowSight Measuring Instruments?
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  Regina GouldSep 8, 2025
  If INSPIRE™ fails to launch on Luminex Measuring Instruments, and the splash screen is not responding, press Ctrl-Alt-Delete on the keyboard, open the task manager, select INSPIRE™ for FlowSight® Software, and press end task. Wait 60 seconds and try restarting INSPIRE™. If there is a loss of communication between the computers and instrument, shut down the computer and power off the instrument. Verify all computers are off, then power on the instrument and then the computer. Wait 5 minutes and try launching INSPIRE™.
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Christopher CarpenterSep 5, 2025
What to do if Luminex Amnis FlowSight will not pass ASSIST?
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  Daniel MunozSep 5, 2025
  If the instrument will not pass ASSIST on Luminex Measuring Instruments, it could be due to several reasons. First, check if the incorrect template is loaded; if so, go to the file drop down and select 'load default template,' then re-run ASSIST. If calibration particles are not running properly, and the beads are diluted or clumped, try running a fresh tube of beads. Finally, if there is a calibration and/or test failure, re-run the test by clicking in the box next to the test and pressing the start button in the popup window. If the test fails three times in a row, contact Luminex Technical Support.

Summary

Chapter 1: General Information and Safety

Electrical Safety

Covers electrical hazards, grounding, and safety precautions for the instrument.

Laser Safety

Details laser classification, accessible radiation, and warning labels for laser safety.

Biological Safety

Addresses biohazard levels, sample handling, and disposal of biological materials.

Spare Parts

States that only Luminex-trained technicians can service the instrument.

Explanation of Symbols

Chapter 2: Experimental Design

Amnis FlowSight Fluorochrome Charts

Provides a chart of recommended fluorochromes for the Amnis FlowSight system.

Chapter 3: Using the Amnis FlowSight Imaging Flow Cytometer

Operating the FlowSight System

Outlines daily operation including initialization, calibration, sample runs, and shutdown.

FlowSight Software User Interface

Describes the layout and components of the FlowSight software interface.

Instrument Control Panel

Details the tools for controlling instrument operation, data acquisition, and status.

Reagents

Lists and describes recommended reagents for optimizing instrument performance.

Daily Startup and Calibration

Provides step-by-step instructions for starting up and calibrating the instrument.

Data Acquisition

Guides users on acquiring experiment data files after system calibration.

Collecting Compensation Files

Explains how to collect compensation samples using the wizard or manually.

Setting Image Display Properties

Describes methods for setting image display properties for visualization.

Setting up the Work Area

Guides on creating scatterplots and histograms in the analysis area.

Quick Start Guide to System Operation

Provides a condensed overview of the system's operational steps.

AutoSampler Option

Explains how to use the AutoSampler for unattended operation of 96-well plates.

Define the Plate

Details the process of creating a Well Plate Definition for the AutoSampler.

Start the AutoSampler

Guides on initiating the unattended plate run using the AutoSampler.

Menus

Describes functions accessible from the INSPIRE software menu bar.

Compensation Menu

Explains operations related to creating, loading, viewing, and editing compensation matrices.

Chapter 4: Troubleshooting

System Troubleshooting

Lists common system issues and potential solutions.

Image Troubleshooting

Addresses problems related to image acquisition and display.

Intensity Troubleshooting

Covers issues with fluorescence intensity appearing too dim or too bright.

Software Troubleshooting

Provides solutions for software-related issues like freezing or slow updates.