Section 4: Experimental Tutorials
• Slowly withdraw the plunger of the plastic filling syringe to draw up the titrant solution till
you see the solution exit through the top filling port.
• Click on the Close Fill Port button as soon as you see the liquid exit the top filling port.
The pipette will lower the plunger of the injection syringe till the white Teflon tip is
completely below the filling port (ca. 4mm). You should here a beep when the movement is
complete.
• Remove the hose of the plastic filling syringe from the filling port of the injection syringe.
• Click on the Purge->ReFill button.
The auto-pipette will depress the plunger of the filling syringe to inject the sample back into
the filling test tube, then raise the plunger to refill the injection syringe. When the movement
is complete the tip of the injection syringe will be positioned to its original position, just
below the filling port. A beep will indicate the completion of the movement.
• Click on the Purge->ReFill button again to repeat the purge/refill action.
The purpose of both purge/refill procedures is to dislodge any air bubbles from inside walls
of the injection syringe (which may have occurred during the first filling) and expel them
back into the titrant solution.
• Carefully remove the pipette from its stand by picking it straight up till the glass barrel of the
injection syringe is above the top part of the pipette stand.
• Carefully move the pipette so that it is directly above the center positioned sample cell access
tube (this is the hole on the right).
53
To Next Page
Loading...