Rotary Microtome HM 340 E
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf 386 200 - English
4-4 HOW TO AVOID MALFUNCTIONS
4-4-1 PREPARATION OF THE SPECIMEN
When preparing specimens, be sure that a suitable
embedding medium, fixation, dehydration and
infiltration time are chosen.
4-4-2 TEMPERATURE OF THE SPECIMEN
Sectioning is carried out at ambient temperature
(excluding frozen sections). If the temperature is
too high, the paraffin softens. Therefore, avoid
heating paraffin specimens by direct exposure to
sunlight or other near sources of heat.
4-4-3 TIGHTENING THE CLAMPING
SCREWS
Tighten all clamping screws and clamping levers
on the knife carrier, specimen holder and specimen
orientation.
4-4-4 SELECTION OF THE KNIFE
Carefully select the required knife material and
profile.
4-4-5 ADJUSTMENT OF THE KNIFE
Take care to adjust the proper clearance angle of
the knife: 5 - 7° for glass knives. For diamond
knives ask the knife manufacturer.
Select a clearance angle adjustment of 5 - 15°
according to the facet angle. Typically, adjust an
angle of 10 - 12 °.
Take care to adjust knife height.
4-4-6 CUTTING SPEED
Take care to select proper cutting speed.
General Rule: The harder the material, the slower
the cutting speed!
4-4-7 TRIMMING
Take care in bringing knife and specimen together.
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