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Nikon ECLIPSE E800 User Manual

Nikon ECLIPSE E800
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4.
Operation
of
Each
Part
Condenser
aperture
diaphragm
The
aperture
diaphragm
is
important
because
it
is
related
to
the
resolution,
contrast,
depth
of
focus,
and
brightness
of
the
optical
image.
Turning
the
condenser
aperture
diaphragm
ring
changes
the
size
of
the
aperture
diaphragm.
As
the
aperture
diaphragm
is
stopped
down,
resolution
and
brightness
are
reduced
while
contrast
and
depth
of
focus
are
increased.
Conversely,
as
the
aperture
diaphragm
is
opened,
resolution
and
brightness
are
increased
while
contrast
and
depth
of
focus
are
reduced.
It
is
not
possible
to
adjust
one
pair
of
characteristics
without
affecting
the
other.
Generally,
a
satisfactory
image
with
appropriate
contrast
can
be
obtained
with
an
aperture
setting
that
is
70%
to
80%
of
the
objective
numerical
aperture.
If
the
aperture
diaphragm
is
stopped
down
too
far,
the
resolution
is
reduced;
therefore,
ex¬
cept
when
viewing
a
nearly
transparent
specimen,
we
do
not
recommend
stopping
down
the
aperture
to
less
than
60%
of
the
numerical
aperture
of
the
objective.
I
Adjusting
the
size
of
the
aperture
diaphragm
according
to
the
condenser
scale
Because
the
condenser
scale
indicates
the
numerical
aperture,
adjust
the
aperture
dia¬
phragm
ring
according
to
the
scale.
Normally,
the
index
on
the
aperture
diaphragm
ring
should
be
aligned
with
the
scale
line
corresponding
to
70%
to
80%
of
the
numerical
aperture
of
the
objective.)
Adjusting
the
size
of
the
aperture
diaphragm
by
using
a
centering
telescope
(sold
separately)
Remove
one
of
the
eyepieces
and,
using
a
special
adapter
(sold
separately),
mount
a
centering
telescope
in
its
place.
Turn
the
aperture
diaphragm
ring
to
stop
down
the
aper¬
ture
diaphragm
as
far
as
possible.
While
holding
down
the
milled
part
of
the
telescope,
turn
the
eyepiece
of
the
centering
telescope
and
focus
on
the
aperture
diaphragm
Turn
the
aperture
diaphragm
ring
to
adjust
the
size
of
the
aperture
diaphragm.
(Nor¬
mally,
the
aperture
diaphragm
should
be
adjusted
so
that
it
is
about
70%
to
80%
of
the
size
of
the
view’
field.)
Remove
the
centering
telescope
and
the
adapter,
and
then
re-install
the
eyepiece.

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Nikon ECLIPSE E800 Specifications

General IconGeneral
BrandNikon
ModelECLIPSE E800
CategoryMicroscope
LanguageEnglish

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