Digital Microscopy Center, University of Washington
July 29, 2016 1
Quick Reference Guide to Using the Nikon Optiphot
(Details begin on following page)
Start-up
1. Epi-fluorescence.
1.1. Turn on the xenon lamp before anything else
1.1.1. Power supply on shelf above microscope
1.1.2. Press black rocker switch
1.1.3. Press the orange button momentarily, and release
1.2. Turn on the microscope lamp
1.2.1. Green push button at left, front base of microscope
1.3. Select your objective lens;
1.4. Select desired epi-fluorescent filter with slider over objective turret
1.5. Open fluorescent shutter
1.6. Set the trinocular beamsplitter to direct the image to the eyepieces;
2. Brightfield.
2.1. Turn on the microscope lamp
2.1.1. black push button at left, front base of microscope
2.2. Select your objective lens;
2.3. Set the trinocular beamsplitter to direct the image to the eyepieces;
2.4. Select “DIA” on filter slider;
2.5. Adjust lamp intensity with slider across base of microscope
2.6. Focus on the specimen
2.7. Set Koehler illumination
2.8. See following pages for use of condenser for darkfield and phase contrast.
Shut down
3. Turn off the equipment.
3.1. Switch off the xenon lamp
3.1.1. Lamp must be on for at least 30 minutes
3.2. Turn off the transmitted lamp
3.3. Remove slide
3.4. Clean any immersion oil or mounting medium from lens and stage
Did you record your usage on the Nikon reservation calendar?
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