Nikon A1+ User Manual

1
Title: Firefly Confocal (Nikon A1+) User Manual
Date of first issue:
23/10/2015
Date of review:
28/08/2017
Version:
Admin
For assistance or to report an issue
Office: CG.07 or CG.05
Email: Igmm-imaginghelpdesk@igmm.ed.ac.uk
Website: www.igmm-imaging.com
Download a PDF copy of manual:
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Manuals\Confocal\Firefly
Facility Usage Policy
1. You must have the relevant Risk Assessment/COSHH form for the
work you are undertaking before using imaging facility resources
2. Users must be trained before using facility equipment
3. Please leave the microscope clean and tidy for the next user
4. Please report any issue, even if it seems minor, to facility staff
5. Any clinical waste must be placed in the orange bins provided

Nikon A1+ Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Scanning Method	Galvanometer-based scanning
Objective Turret	Motorized
Dimensions	Varies by configuration
Weight	Varies by configuration
Scan Speed	Up to 30 fps (512 x 512 pixels)
Objective Lenses	Compatible with Nikon CFI series objectives
Frame Rate	30 frames per second

Summary

Assistance and Facility Usage Policy

Facility Usage Policy

Guidelines for using imaging facility resources, including training, cleanliness, and reporting issues.

System Startup Procedures

Device Identification and Setup

Steps for powering on and configuring essential microscope components and related equipment.

List of Microscope Devices

System Shutdown

Mounting a Sample

Finding the Sample by Eye

Nis-Elements Software Layouts

Selecting a Confocal Channel Setup

Adding a Brightfield Channel

Capture an Image

Pre-Scan Acquisition Settings

Optimising Scan Settings for Image Quality

Compact GUI Diagram and Controls

Image Saturation Adjustment

Capture a Single XY Image

Image Window Options

Adding a Scale Bar

Optical Zoom for Image Capture

Z Stack Acquisition Process

Z Stack Viewing Options

XYZT Experiment Setup

Multi-position XYZT Experiments

Tile-Scan Experiments

Basic and Advanced Tiling Setup

Configuring tiling for montage images and defining scan areas.

FRAP/Bleaching Experiments

Appendix: Image Saving and Exporting

Exporting Images in TIFF Format

Detailed steps for exporting images in TIFF format with various compatibility options.

Kohler Illumination Procedure

Perfect Focus System (PFS) Operation

Frequently Asked Questions

Improving Image Quality (Noise)

Troubleshooting noisy images by adjusting gain, averaging, and scan speed.

Troubleshooting: Cannot See Sample

Steps to resolve issues when no sample is visible through the microscope eyepieces.

Troubleshooting: Sample Field of View

Methods for capturing areas larger than the current field of view, like tiling.