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Nikon Alphaphot User Manual

Nikon Alphaphot
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Nikon Alphaphot Specifications

General IconGeneral
BrandNikon
ModelAlphaphot
CategoryMicroscope
LanguageEnglish

Summary

Cautions in Handling

Avoid Sharp Knocks

Handle the microscope gently to prevent damage from impacts.

Locations of Microscope

Avoid conditions like dust, vibration, high temperature, moisture, or direct sunlight.

Replacing the Lamp Bulb or Fuse

Turn off power and disconnect cord before replacing lamp or fuse.

Dirt on the Lens

Keep lenses clean to ensure clear observation of specimen images.

Care and Maintenance

Cleaning the Lenses

Clean lenses with soft brush or gauze; use alcohol or xylene cautiously for marks.

Cleaning the Painted Surfaces

Avoid organic solvents for cleaning painted and plastic parts.

Never Attempt to Dismantle

Do not dismantle the instrument to maintain operational efficiency and accuracy.

When Not in Use

Cover the instrument with a vinyl cover and store in a dry, fungus-free place.

Periodical Checking

Recommend periodic checks to maintain instrument performance.

Nomenclature

Assembly

Lamp

Instructions for installing either halogen or tungsten lamps.

Eyepiece Tube

Mount the eyepiece tube onto the microscope arm.

Eyepiece

Insert the eyepiece into the eyepiece sleeve.

Objective

Lower the stage and screw the objective into the revolving nosepiece.

Mechanical Stage

Attach the mechanical stage to the microscope stage securely.

Filter ($33)

Insert the filter into the filter holder and then into the condenser.

Condenser

Slide the condenser into its carrier and tighten the clamp screw.

Microscopy

Lighting the Lamp

Turn the dial to achieve adequate brightness for observation.

Setting the Specimen

Place the specimen on the stage with the cover glass facing upward.

Focusing with 10x Objective

Use coarse and fine focus knobs to achieve a sharp image.

Adjusting the Interpupillary Distance

Adjust for both right and left images to merge into one.

Adjusting the Eyesight Difference

Adjust focus for each eyepiece individually using focus knobs and diopter ring.

Centering the Field Diaphragm

Center the field diaphragm image within the field of view.

Changing Over to the Objective to be Used

Select and align the desired objective on the revolving nosepiece.

Adjusting the Aperture Diaphragm

Adjust aperture to optimize image contrast and resolution.

Adjusting the Field Diaphragm

Adjust field diaphragm to match the field of view for optimal contrast.

Oil-Immersion Observation

Apply immersion oil between coverglass and objective for high magnification.

Adjustments So Far as Expedient for Operators

Adjustment of Brightness of Image

Adjust resistance compensator for optimal lamp brightness.

Adjustment of Tension of the Coarse Focus Knob

Adjust coarse focus knob tension using a hexagonal wrench.

Height Adjustment by Leveling Foot Screw

Level the microscope using the foot screw on the base.

Optical Features of Alphaphot

Combination of Eyepiece 10x (Field Number 18) with CF E Achromat and CF Achromat Objectives

Details on objective and eyepiece combinations and their optical performance.

Terms Describing the Optical Features of Microscopes

Explanation of terms like magnification, numerical aperture, and resolving power.

Trouble Shooting Table

Optical

Troubleshooting optical issues like darkness, dirt, or poor image quality.

Manipulation

Troubleshooting issues related to specimen focus, stage movement, and image fusion.

Electrical

Troubleshooting electrical problems like lamp not lighting or unstable brightness.

Electric Specifications

Technical specifications for power source, lamp bulb, and fuse for different sets.

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