Nikon eclipse lv150 User Manual

Industrial Microscope
ECLIPSE LV150/LV150A
Instructions
M350 E 05.4.NF.1

Nikon eclipse lv150 Specifications

General

Brand	Nikon
Model	eclipse lv150
Category	Microscope
Language	English

Summary

Safety Symbols and Warnings

Manual Safety Symbols

Explains warning and caution symbols used within the instruction manual for user safety.

Equipment Safety Symbols

Details symbols found on the equipment, such as caution for heat, to ensure safe operation.

General Safety Warnings

Product Use, Disassembly, Power, and Light Source Safety

Covers intended use, warnings against disassembly, power supply ratings, cord usage, and specified light sources for safety.

General Safety Warnings

External Light, Heat, UV, and Air Vent Safety

Addresses safety for non-specified light sources, heat hazards, air vent importance, and ultraviolet light protection.

Operational Cautions

Handling, Installation, and Moving Cautions

Provides cautions on gentle handling, avoiding moisture, electromagnetic interference, proper installation, and safe movement.

Operational Cautions

Assembly, Lamp, and Filter Cube Cautions

Details cautions for microscope assembly, lamp replacement, and safe handling of filter cubes.

Diagrams and Layouts

Microscope Layout Diagrams

Visual representations of the microscope's layout and key dimensions for reference.

Ergonomics and Posture

Recommended Operating Postures

Illustrates recommended postures for comfortable and strain-free microscope operation for different body types.

Microscope Part Identification: LV-UEPI

Main Components with LV-UEPI Illuminator

Identifies and labels the main components of the microscope when using the LV-UEPI illuminator.

Microscope Part Identification: Controls

Overview of Operational Controls and Features

Details and labels the various operational controls and functional parts of the microscope.

Microscope Part Identification: LV-UEPI2

Main Components with LV-UEPI2 Illuminator

Identifies and labels the main components when the LV-UEPI2 illuminator is used.

Microscope Part Identification: Controls (LV-UEPI2)

Operational Controls for LV-UEPI2 Configuration

Details and labels the operational controls specific to the LV-UEPI2 illuminator setup.

Microscope Diagrams: Rear View

Rear View of Microscope Components

Shows the rear side of the microscope and labels its connection ports and indicators.

Microscopy Techniques Overview

Microscopy Types and Required Accessories

Summarizes different microscopy methods (bright-field, dark-field, DIC, etc.) and their required optional accessories.

Microscopy: Bright-Field (LV-UEPI)

Bright-Field Microscopy Procedure

Step-by-step guide for performing bright-field microscopy using the LV-UEPI illuminator.

Microscopy: Bright-Field (LV-UEPI2)

Bright-Field Microscopy Procedure

Step-by-step guide for performing bright-field microscopy with the LV-UEPI2 illuminator.

Microscopy: Dark-Field (LV-UEPI)

Dark-Field Microscopy Procedure

Instructions for dark-field microscopy using the LV-UEPI illuminator.

Microscopy: Dark-Field (LV-UEPI2)

Dark-Field Microscopy Procedure

Instructions for dark-field microscopy using the LV-UEPI2 illuminator.

Microscopy: DIC (LV-UEPI)

DIC Microscopy Procedure

Step-by-step guide for Differential Interference Contrast (DIC) microscopy with LV-UEPI.

Microscopy: DIC (LV-UEPI2)

DIC Microscopy Procedure

Step-by-step guide for Differential Interference Contrast (DIC) microscopy with LV-UEPI2.

Microscopy: Polarization (LV-UEPI)

Simplified Polarization Microscopy Procedure

Procedure for performing simplified polarization microscopy using LV-UEPI.

Microscopy: Polarization (LV-UEPI2)

Simplified Polarization Microscopy Procedure

Procedure for performing simplified polarization microscopy using LV-UEPI2.

Microscopy: Sensitive Polarization (LV-UEPI2)

Sensitive Polarization Microscopy Procedure

Steps for sensitive polarization microscopy with the LV-UEPI2 illuminator.

Microscopy: Epi-Fluorescence (LV-UEPI2)

Epi-Fluorescence Microscopy Procedure

Guide for setting up and performing epi-fluorescence microscopy with LV-UEPI2.

Microscope Operation: Illumination

Illumination System Controls

Details on controlling brightness, turning the lamp on/off, and understanding the power indicator.

Microscope Operation: Focusing

Focus Knobs and Stage Movement

Explains the relationship between focus knobs, stage movement, and torque adjustment for precise focusing.

Microscope Operation: Focusing

Coarse Focus Stopper Mechanism

Describes how to use the coarse focus stopper to limit stage vertical movement and its example usage.

Microscope Operation: Eyepieces

Eyepiece Tube and Adapters

Details optical path selection, vertical tube adapters, and binocular part adjustments for eyepieces.

Microscope Operation: Eyepieces

Diopter and Interpupillary Distance Adjustment

Guides on adjusting diopter for individual eyesight and interpupillary distance for binocular viewing.

Microscope Operation: Illumination Controls

Field Diaphragm Adjustment and Centering

Explains how to adjust and center the field diaphragm for optimal illumination and image contrast.

Microscope Operation: Illumination Controls

Aperture Diaphragm Adjustment

Details on adjusting the aperture diaphragm for resolution, contrast, and depth of focus.

Microscope Operation: Illumination and Microscopy Selection

Illumination and Microscopy Selection Controls

Describes controls for selecting illumination types (BF/DF) and microscopy modes (FL1, S, FL2).

Microscope Operation: Stage

Stage Movement and Usage

Explains the 6x6 stage's coarse/fine modes, stage movement, and slide glass usage.

Microscope Operation: Nosepiece

Motorized Nosepiece Operation

Instructions on using the motorized nosepiece to change objectives, including mounting precautions.

Microscope Operation: Polarization

Polarizer Slider Usage and Orientation

Details on inserting, removing, and orienting the polarizer slider for polarization microscopy.

Microscope Operation: Polarization

Lambda Plate Slider Operation

Instructions for inserting and removing the lambda plate slider for sensitive polarization microscopy.

Microscope Operation: Polarization

Analyzer Slider Insertion and Removal

Guide on how to insert and remove the analyzer slider for polarization and DIC microscopy.

Microscope Operation: DIC

DIC Slider and Prism Selection

Details on attaching, using the DIC slider, and selecting the correct prism position for DIC microscopy.

Microscope Operation: Fluorescence

Filter Cubes for Fluorescence

Information on selecting and using filter cubes for epi-fluorescence microscopy with the LV-UEPI2.

Microscope Operation: Fluorescence

Excitation Filter Selection for Fluorescence

Explains how to choose excitation filters based on sample characteristics and desired fluorescence.

Microscope Operation: Fluorescence

Barrier Filter Selection for Fluorescence

Guide on selecting barrier filters (LP and BP) to improve fluorescence image observation.

Microscope Operation: Fluorescence

Excitation Light Balancer Usage

Details on attaching and using the excitation light balancer for multi-color fluorescence observation.

Microscope Operation: Fluorescence

Excitation Light Balancer Specifications

Provides detailed specifications and transmittance data for the excitation light balancer.

Microscope Assembly: Preparation

Assembly Safety, Tools, Location, and Component Compatibility

Important safety warnings, required tools, installation site selection, and illuminator/light source compatibility.

Microscope Assembly: Stage and Nosepiece

Attaching Stage, Holder, and Manual Nosepiece

Step-by-step instructions for attaching the stage, holder, and assembling the manual nosepiece.

Microscope Assembly: Sub-stages

Sub-stage Attachment Procedures

Details on attaching the standard sub-stage and the optional sub-stage 2 for different stage configurations.

Microscope Assembly: Stage and Nosepiece Details

Stage and Manual Nosepiece Assembly Steps

Specific steps for attaching 6x6 and 3x2 stages, and assembling the manual nosepiece.

Microscope Assembly: Motorized Nosepiece

Motorized Nosepiece Assembly and Removal

Instructions for assembling and removing the motorized nosepiece, including cable connection.

Microscope Assembly: Illuminator

Attaching the Illuminator Unit

Steps for mounting the illuminator main unit, including attaching the UV shield for LV-UEPI2.

Microscope Assembly: Sliders and Filters

Slider and Filter Installation Procedures

Instructions for inserting sliders and attaching filter sliders with filters into the illuminator.

Microscope Assembly: Fluorescence Cubes

Filter Cube Installation for Fluorescence

Steps for installing filter cubes for epi-fluorescence microscopy and applying excitation method stickers.

Microscope Assembly: Lamphouse

Lamphouse Attachment Procedures

Procedures for loosening clamp screws, mounting the lamphouse, and securing it with screws.

Microscope Assembly: Lamp and Light Sources

Lamp Replacement and External Light Source Attachment

Guide for replacing the lamp and attaching fiber adapters and external light sources.

Microscope Assembly: Adapters and Filters

Fiber Adapter, Compensation Filter, and External Source Connection

Instructions for attaching fiber adapters, compensation filters, and connecting external light sources.

Microscope Assembly: Optics and Eyepieces

Attaching Eyepiece Tube, Eyepieces, and Objectives

Steps for installing the eyepiece tube, eyepieces, and objectives onto the microscope.

Microscope Assembly: Risers

Attaching Eye Level and Column Risers

Instructions for attaching optional eye level and column risers to adjust height and clearance.

Microscope Assembly: Connections and Anti-static

Power, RS-232C, Accessories, and Anti-static Measures

Covers connecting the power cord, RS-232C, installing accessories, and anti-static considerations.

Microscope Assembly: Earthquake Protection

Earthquake Countermeasures

Recommendations for securing the microscope against seismic activity using brackets and anti-vibration tables.

External Communication Control: Basics

Communication Method and Connector Specifications

Details the asynchronous serial communication method and RS-232C connector pin assignments.

External Communication Control: Data Handling

Cable Specs, Communication Parameters, and Formats

Covers cable requirements, communication parameters (baud rate, data length), and data formats for external control.

External Communication Control: Data Handling

Sending Data Format and Communication Sequence

Defines the sending data format and illustrates the communication sequence for external control.

External Communication Control: Status Modes

Communication Status Output Modes

Describes different modes for communication status output, including external light source shutter control.

Troubleshooting: Viewing and Control Systems

Viewing and Control System Problems

Addresses issues like invisible viewfields, incorrect brightness, and improper component seating.

Troubleshooting: Image Quality and Brightness

Image Quality and Brightness Troubleshooting

Solves problems related to image appearance, color tinting, brightness levels, and overall viewing quality.

Troubleshooting: Mechanical and Optical Issues

Mechanical and Optical Operation Issues

Addresses issues with objective collision, stage movement, focus, eyepieces, and specific microscopy mode problems.

Troubleshooting: Electrical and Nosepiece

Electrical and Nosepiece Operation Issues

Covers electrical problems like lamp failure, flickering, and nosepiece rotation malfunctions.

Microscope Care and Maintenance: Daily Practices

Daily Care and Maintenance Practices

Guidelines for regular cleaning and upkeep to maintain optical performance and prevent damage.

Microscope Care and Maintenance: Procedures

Cleaning, Storage, and Inspection Procedures

Detailed procedures for cleaning lenses, parts, proper storage, and recommended regular inspections.

Microscope Specifications: Technical Details

Technical Specifications Overview

Technical specifications including model, optical system, illumination, focusing, and input/power details.

Microscope Specifications: Compliance

Safety Standards and Compliance

Lists safety certifications (TUV, UL, FCC, IC, EMI) and compliance standards for the microscope.