17
II. Microscopy
When configured with the LV-UEPI2
6
x
6
ST
AG
E
JAP
AN
A
.
STOP
F
.
STOP
JAP
A
N
FL1
S
FL2
BF
DF
1
0
0
2
0
0
1
0
0
I
N
O
U
T
L
V
-
T
T
2
6
x
6
STAGE
JAP
AN
A
.
STOP
F
.
STOP
JAPA
N
FL1
S
FL2
BF
DF
1
0
0
2
0
0
1
0
0
IN
O
U
T
L
V
-T
T
2
4
5
2
6
4
5
3
3
1
1
2
Power switch
6
7
8
Adjust the
brightness.
ND filter
(p.26)
Adjust the
brightness.
Brightness control
knob (p.26)
Lower the stage
as far as it will go.
Coarse focus knob
(p.27)
Adjust the
brightness.
Brightness control
knob (p.26)
Adjust
the brightness.
ND filter
(p.26)
Image of field diaphragm
Viewfield
Objective’s
pupil
Image of
aperture
diaphragm
Adjust to 70 to 80% of
the objective’s N.A.
Aperture diaphragm
(p.32)
Adjust to circumscribe
the viewfield.
Field diaphragm (p.31)
Select the
10x objective.
On the LV150A,
use the nosepiece
rotation buttons. (p.35)
Raise the levers.
Push in the
NCB11 filter.
To fully open the field and
aperture diaphragms.
(p.31 and p.32)
To compensate
color temperature.
(p.26)
Select the desired
magnification.
On the LV150A,
use the nosepiece
rotation switch.
(p.35)
Finely adjust
the focus.
Coarse/fine focus
knob (p.27)
Push in.
Binocular eyepiece: 100% (P.29)
Turn the
microscopy
selection knob.
BF (bright-field) (P.33)
2. Set the microscope for
bright-field microscopy.
If accessories for DIC or
polarization microscopy
(*1 to *3) are in place,
pull them out of the
optical path.
1. Turn on the power.
3. Place the sample on the
stage and focus on it.
(p.27)
4. Adjust the angle of the
binocular part. (p.29)
5. Adjust the diopter. (p.30)
6. Adjust the interpupillary
distance. (p.30)
7. Change the magnification
and observe the sample.
Hint:
It may be difficult to
focus on a sample with
small contrast, such on a
polished surface. In a
case like this, stop down
the field diaphragm so
that its image can be
seen in the viewfield,
and try to focus on the
rim of the diaphragm
image. When the rim is
in focus, the sample is in
focus just as well.
*2
*3
*1
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