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Nikon Eclipse LV100DA-U User Manual

Nikon Eclipse LV100DA-U
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Universal Design Microscope UDM
ECLIPSE LV100DA-U
Instructions
M421 E 07.5.NF.1 (2/3)

Table of Contents

Questions and Answers:

Nikon Eclipse LV100DA-U Specifications

General IconGeneral
TypeUpright microscope
Optical SystemCFI60 infinity optical system
Light Source100W halogen lamp
StageRectangular mechanical stage
CameraOptional
ObjectivesCFI Plan Apochromat series
IlluminationTransmitted and reflected
FocusingCoarse and fine focusing
Observation MethodBrightfield, darkfield, DIC, fluorescence
Eyepiece10x
Camera PortOptional

Summary

Symbols and Warnings

Product Symbols and Heat Caution

Explains symbols used on the product, including a "Caution for heat" marking.

Critical Safety Warnings

Intended Use and Disassembly Prohibition

Specifies intended use and warns against disassembling the microscope to prevent hazards.

Power Supply and Cord Requirements

Details power supply ratings and power cord specifications for safe operation.

Specified Light Sources

Lists compatible light sources and accessories for the microscope system.

External Light Source and UV Hazards

Addresses usage of external light sources and potential UV light exposure hazards.

Heat and Air Vent Precautions

Warns about heat from the light source and the importance of clear air vents.

Operating Motorized Units Safely

Cautions regarding safe operation of motorized components to prevent injury.

General Cautions and Handling Procedures

Careful Handling and Environmental Considerations

Emphasizes careful handling, avoiding moisture, and electromagnetic wave interference.

Installation and Moving Precautions

Guidelines for proper installation location and safe methods for moving the microscope.

Assembly and Maintenance Cautions

Cautions during assembly, lens care, and lamp replacement procedures.

Filter Cube and Software Setup

Notes on handling filter cubes and the necessity of software setup after assembly.

Microscope Component Identification

Front Left and Right Views

Diagrams and labels identifying components on the front left and right sides of the microscope.

Operation Panel and Connectors

Details the functions of the operation panel and the various connectors.

Rear View Component Identification

Diagrams and labels identifying components on the rear of the microscope.

Microscopy Method Setup

Initial Setup and Interlock Control

Explains the initial setup using LVSetup and how interlock control affects microscopy.

Microscopy Methods Overview

A list and summary of available microscopy methods and required accessories.

Epi Illumination - Bright-field Microscopy

Setting Up for Bright-field Observation

Step-by-step guide for setting up bright-field microscopy with epi illumination.

Focusing and Adjusting Brightness

Procedures for specimen focus, brightness adjustment, and eyepiece settings.

Epi Illumination - Advanced Microscopy Techniques

Dark-field Microscopy Setup

Steps for setting up dark-field microscopy and returning to bright-field.

Polarization Microscopy Setup

Procedures for setting up polarization microscopy and returning to bright-field.

Epi Illumination - Differential Interference Contrast (DIC) Microscopy

Senarmont Method DIC Setup

Setup procedures for Senarmont method DIC microscopy.

Prism Slide Method DIC Setup

Setup procedures for Prism Slide method DIC microscopy.

Returning to Bright-field from DIC

Steps to revert to bright-field microscopy after DIC observation.

Epi-fl Microscopy Procedures

Setting Up for Epi-fl Observation

Steps to configure the microscope for epi-fl microscopy.

Returning to Bright-field/Dark-field

How to return to bright-field or dark-field from epi-fl microscopy.

Diascopic Illumination - Bright-field Microscopy

Setting Up for Diascopic Bright-field

Step-by-step guide for setting up bright-field microscopy with diascopic illumination.

Specimen Focus and Observation

Procedures for specimen focus and observation in diascopic bright-field.

Diascopic Illumination - Polarization Microscopy

Setting Up for Diascopic Polarization

Steps for setting up polarization microscopy with diascopic illumination.

Returning to Bright-field

How to return to bright-field microscopy from diascopic polarization.

Diascopic Illumination - Dark-field Microscopy

Setting Up for Diascopic Dark-field

Steps for setting up dark-field microscopy with diascopic illumination.

Returning to Bright-field

How to return to bright-field microscopy from diascopic dark-field.

Diascopic Illumination - Phase Contrast Microscopy

Setting Up for Diascopic Phase Contrast

Steps for setting up phase contrast microscopy with diascopic illumination.

Returning to Bright-field

How to return to bright-field microscopy from diascopic phase contrast.

Diascopic Illumination - DIC Microscopy

Setting Up for Diascopic DIC

Steps for setting up DIC microscopy with diascopic illumination.

Returning to Bright-field from Diascopic DIC

How to return to bright-field microscopy from diascopic DIC.

Microscope Operation Basics

Powering On and Off the Microscope

Detailed steps for powering the microscope on and off, including initialization.

External Power and Light Source Operation

Procedures for operating external power supplies and light sources.

Initial Microscope Setup

Initial Microscope Setup Procedure

Guide for initial setup using LVSetup software and PC connection.

Microscopy Method Selection

Switching Illumination Sources

How to switch between episcopic and diascopic illumination using panel switches.

Changing Microscopy Methods (Epi)

How to select different microscopy methods using the CUBE switch for epi illumination.

Microscopy Method Summaries

Episcopic Illumination Methods

A table summarizing episcopic microscopy methods with details.

Diascopic Illumination Methods

A table summarizing diascopic microscopy methods with details.

Interlock Control Configuration

Interlock Control for Microscopy Methods

Explains interlock control linking devices to microscopy and objective settings.

User Offset Management

How to modify and reset user offset settings for illumination and aperture.

Illumination Control

Controlling Illumination On/Off

Using EPI and DIA switches for illumination control and brightness.

External Light Source and Antiglare Function

Operating external sources and the antiglare function.

Brightness Control and Interlock

Brightness Adjustment Methods

Using brightness switches and interlock controls for illumination brightness.

Objective and Filter Management

Objective Handling and Switching

How to rotate the nosepiece, interlock control, and switching restrictions.

Filter Usage and Types

Lists and describes filters for both episcopic and diascopic illumination.

Stage and Focusing Mechanisms

Stage Operation and Types

How to operate the stage, use glass slide holders, and center the stage.

Focus Knob Operation and Adjustment

Details coarse/fine focus knobs, stiffness adjustment, and stopper function.

Eyepiece and Viewing Adjustments

Eyepiece Tube and Path Selection

Optical path selection, adapter use, and eyepiece tube centering.

Diopter and Interpupillary Distance Adjustment

Procedures for adjusting eyepiece diopters and interpupillary distance.

Episcopic Illumination Adjustments

Field Diaphragm Control

Adjusting the field diaphragm for specimen illumination and centering.

Aperture Diaphragm Control

Adjusting the aperture diaphragm for resolution/contrast and centering.

Interlock Control and Reset for Aperture

How interlock control affects the aperture diaphragm and how to reset settings.

Diascopic Illumination Adjustments

Condenser Focusing and Centering

Steps for focusing and centering the condenser for diascopic illumination.

Field and Aperture Diaphragm Control (Diascopic)

Adjusting field and aperture diaphragms for diascopic illumination.

Condenser Operation and Selection

Operating slide and universal condensers, and selecting condenser modules.

Polarizer Slider Installation and Operation

Episcopic Polarizer Slider Usage

Details on types and usage of polarizer sliders for episcopic illumination.

Diascopic Polarizer Installation and Adjustment

How to attach and adjust polarizers for diascopic illumination.

Analyzer Slider Installation and Usage

Analyzer Slider Operation

Steps for placing, removing, and orienting the analyzer slider.

PA Block Usage and Installation

PA Block Functionality and Structure

Explains the PA block's function, structure, and installation.

Lambda Plate Slider for Episcopic Illumination

Lambda Plate Slider Operation

Steps for inserting and removing the lambda plate slider for episcopic illumination.

Lambda Plate Slider for Diascopic Illumination

Lambda Plate Slider Operation (Diascopic)

Steps for attaching and operating the lambda plate slider for diascopic illumination.

DIC Prism Installation and Usage

Episcopic DIC Prism Setup (Senarmont)

Selecting, attaching, and setting up DIC prisms for Senarmont method.

Episcopic DIC Prism Setup (Prism Slide)

Selecting, attaching, and setting up DIC prisms for Prism Slide method.

Diascopic DIC Prism Setup

Selecting and installing DIC prisms for diascopic illumination.

Prism Combinations with Objectives

Table showing optimal prism combinations with objectives for contrast/resolution.

Fluorescence Microscopy and Assembly

Filter Cubes for Fluorescence

Details on filter cubes, light sources, and EX/BA filter selection for epi-fl microscopy.

Excitation Light Balancer Usage

How to use the excitation light balancer and its objective compatibility.

General Assembly Precautions

Warnings and cautions before starting assembly and the importance of software setup.

Required Tools and Installation Location

Lists assembly tools and guidelines for selecting an installation location.

Illuminator and Light Source Combinations

Lists compatible illuminators and light sources, noting UL listing.

Microscope Assembly Steps

Stage Unit Attachment

Instructions for attaching various stage types, glass holders, and condenser plates.

Condenser Attachment and Selection

Steps for attaching and selecting the appropriate condenser based on stage type.

Motorized Nosepiece Installation

Steps for attaching the motorized nosepiece (LV-NU5AI).

DIC Prism Installation

Attaching DIC prisms (objective and condenser) for epi and diascopic illumination.

Epi Illuminator (LV-UEPI2A) Mounting

Mounting the LV-UEPI2A unit, connecting cables, and installing light shields.

Slider Installation (Analyzer, Polarizer)

How to insert sliders into the LV-UEPI2A.

Filter Slider and Filter Installation

How to install filter sliders and filters into the epi illuminator.

Filter Cube Installation (Epi-fl)

Steps for installing filter cubes or PA blocks into the LV-UEPI2A turret.

Lamp House Attachment and Lamp Replacement

Procedures for attaching the lamp house and replacing lamps.

External Power Supply Connection

Connecting the TE2-PS100W for simultaneous illumination.

External Light Source Integration

Connecting external light sources, adapters, and RS-232C cables.

Compensation Filter Attachment

Installing the compensation filter with the HG fiber adapter.

Double Light Source Adapter Installation

Attaching the adapter for switching between light sources.

Eyepiece Tube and Objective Mounting

Installing the eyepiece tube, eyepieces, and objectives.

Diascopic Polarizer Installation

How to attach and adjust polarizers for diascopic illumination.

Eye Level and Column Riser Installation

Attaching optional risers to adjust eyepiece height and column riser.

PC Connection and Setup

Connecting the PC for microscope setup using LVSetup software.

DS-L2 Controller Connection

Connecting the DS-L2 controller for camera control.

Power Cord Connection and Options

Connecting the power cord and notes on anti-static features.

Troubleshooting Common Issues

Viewfield and Image Quality Problems

Troubleshooting for viewfield visibility, brightness, contrast, and resolution.

Focus and Image Stability Issues

Solutions for focus problems, image elongation, and specimen drift.

Color, Brightness, and Illumination Issues

Troubleshooting for color tints, brightness control, and illumination problems.

Electrical System Problems

Diagnosing and resolving electrical issues with power, lamps, and controllers.

Component-Specific Troubleshooting

Troubleshooting for LV-UEPI2A and motorized nosepiece specific issues.

Technical Specifications

Microscope (LV100DA-U) Specifications

Detailed technical specifications for the main microscope unit.

Illuminator (LV-UEPI2A) Specifications

Detailed specifications for the LV-UEPI2A epi illuminator.

Nosepiece (LV-NU5AI/NU5A/NU5AC) Specifications

Detailed specifications for the motorized nosepieces.

Power Supply (TE2-PS100W) Specifications

Detailed specifications for the TE2-PS100W power supply.

Operating and Storage Conditions

Environmental requirements for operation, storage, and safety compliance.

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