Nikon Eclipse LV100DA-U - Dark-Field Microscopy under the Dia Illumination

29
II. Microscopy Method
9
Dark-field Microscopy under the Dia Illumination
2
Focus on the specimen with the bright-field microscopy under the diascopic illumination.
(See Pages 26 to 27.)
3
Set the microscope for the dark-field
microscopy under the diascopic illumination.
1 Rotate the condenser turret of the universal
condenser to the “DF” position. (See Page 55.)
2 Press the OBJ. switch on the operation panel and
locate the desired objective into the optical path.
(See Page 43.)
The objective of a magnification of 4x or more
and a numerical aperture of 0.7 or less is usable
to the dark-field microscopy.
3 Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)
4 Adjust the brightness with ND filters.
(See Page 44.)
5 Fully open the field diaphragm and the aperture
diaphragm. (See Page 54.)
4
Return to the bright-field microscopy under the
diascopic illumination.
1 Rotate the condenser turret of the universal
condenser to the “O” position. (See Page 55.)
2 Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)
3 Adjust the brightness with ND filters.
(See Page 44.)
4 Adjust the field diaphragm and the aperture
diaphragm. (See Page 54.)
U
E
P
I2
A
US
B
RS
2
3
2C
L
CN
T
N
D
8
N
C
B
F
.
S
.
J
A
P
A
N
6
96
0
0
1
3
x
2
S
T
A
G
E
J
A
P
A
N
F
.
STO
P
J
A
P
A
N
BF DF FL1 FL2
FL1
FL2
1
0
02
0
0
10
0
IN
OUT
LV
-
T
T
2
OBJ.
CUBE
A.S.
EPI
DIA
EPI
DIA
5
3
1
4
5
2
2
Operation
panel
OBJ. switch
DIA brightness switch
U
E
P
I2
A
US
B
RS
2
3
2C
L
CN
T
N
D
8
N
C
B
F
.
S
.
J
A
P
A
N
696
0
01
3
x
2
S
T
A
G
E
J
A
P
A
N
F
.
STO
P
JA
P
A
N
BF DF FL1 FL2
FL1
FL2
1
0
020
0
1
0
0
IN
OUT
LV
-
T
T2
OBJ.
CUBE
A.S.
EPI
DIA
EPI
DIA
4
2
3
4
1
Operation
panel
DIA brightness switch
1
Attach the accessories required for the dark-field microscopy under the dia illumination.
The following accessories must be attached to perform the simplified/sensitive color dark-field
microscopy under the dia illumination.
• Universal condenser (dry) and dark-field annular diaphragm (See Page 78 and the LV-CUD
instruction manual.), or dark-field condenser (See Page 78.)