Nikon A1+ User Manual

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Table of Contents
System Startup ..................................................................................................................... 3
List of devices ...................................................................................................................... 4
System Shutdown ................................................................................................................ 5
Mounting a sample ............................................................................................................... 5
Finding the sample by eye ................................................................................................... 6
Nis-Elements software layouts ............................................................................................. 7
Selecting a confocal channel setup ..................................................................................... 7
Adding a brightfield channel ................................................................................................. 8
Capture an image ................................................................................................................ 8
Pre-scan acquisition settings ............................................................................................... 9
Optimising scan settings for best image quality ................................................................. 10
Image Saturation ................................................................................................................ 12
Capture a single XY image ................................................................................................ 13
Image Window Options ...................................................................................................... 13
Adding a scale bar ............................................................................................................. 13
Capture images with Optical Zoom .................................................................................... 14
Z Stack Acquisition ............................................................................................................. 15
Z stack viewing options ...................................................................................................... 16
XYZT Experiments ............................................................................................................. 17
Multi-position XYZT Experiments ....................................................................................... 18
Tile-Scan Experiments ....................................................................................................... 19
FRAP/Bleaching Experiments ............................................................................................ 21
Appendix ........................................................................................................................... 24
Saving Images ................................................................................................................... 24
Export images in tiff format ................................................................................................ 24
Kohler Illumination ............................................................................................................. 25
Perfect Focus System (PFS) Operation ............................................................................. 26
Frequently Asked Questions .......................................................................................... 27
1. My images look noisy, what can I do to improve image quality? .................................. 27
2. I can't see anything down the microscope .................................................................... 27
3. I can't fit all of the tissue into the field of view ............................................................... 28

Nikon A1+ Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Scanning Method	Galvanometer-based scanning
Objective Turret	Motorized
Dimensions	Varies by configuration
Weight	Varies by configuration
Scan Speed	Up to 30 fps (512 x 512 pixels)
Objective Lenses	Compatible with Nikon CFI series objectives
Frame Rate	30 frames per second