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Nikon SC - Optical Features and Terms; Key Optical Definitions

Nikon SC
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IV.
OPTICAL
FEATURES
OF
SC
MICROSCOPE
Combination
of
Eyepiece
10xSC
(field
number
18}
with
Objective
SC
series
Objective
Total
Magnifi¬
cation
Numerical
aperture
(N.A.)
Real
viewfield
Depth
of
focus
Resolving
power
Working
distance
(W.D.)
4XSC
40X
0.1
4.5
mm
63.2
Jim
2.8
Jim
28
mm
10XSC
100X
0.25
1.8
mm
10.1
Jim
1.1
Jim
8
mm
40XSC
400X
0.65
0.45mm
0.97jim
0.42jim
0.73mm
100XSC
1000X
1.25
0.18mm
0.33jim
0.22jjm
0.16mm
TERMS
DESCRIBING
THE
OPTICAL
FEATURES
OF
MICROSCOPES
•
Total
magnification
Total
magnification
of
a
microscope
is
the
in¬
dividual
magnifying
power
of
the
objective
multiplied
by
that
of
the
eyepiece.
•
Numerical
aperture
(N.A.I
One
of
the
important
factors
determining
the
efficiency
of
condenser
and
objective.
It
is
represented
by
the
formula:
N.A.
=
n
sin
a.
Where
n
is
the
refractive
index
of
a
medium
{air,
immersion
oil.
etc.)
between
the
objec¬
tive
lens
and
the
specimen
or
condenser,
and
a
is
a
half
of
the
maximum
angle
at
which
the
light
rays
enter
or
leave
the
lens
from
or
to
a
focused
object
point
on
the
optical
axis.
The
larger
the
numerical
aperture,
the
brighter
and
better
resolved
the
image.
•
Resolving
power
Capability
of
discriminating
two
object
points
separated
by
a
minute
distance
on
the
image
the
optical
system
produces,
dius
being
taken
as
a
definition
standard
of
image
resolution.
The
more
minute
such
a
distance,
the
higher
the
resolving
power
of
the
optical
system.
In
relation
to
the
numerical
aperture,
the
re¬
solving
power
is
represented
by
the
value:
X/2
N.A.
where
\
is
the
wavelength
of
light
being
used.
•
Mechanical
tube
length
Length
from
the
attaching
surface
of
the
ob¬
jective
on
the
nosepiece
to
the
top
end
of
the
sleeve
into
which
the
eyepiece
is
inserted.
•
Working
distance
(W.D.)
Clearance
between
the
front
of
the
objective
and
the
upper
surface
of
the
coverglass,
when
the
image
of
a
specimen
is
brought
into
sharp
focus.
•
Real
viewfield
Diameter
of
the
circular
area
of
the
specimen
actually
covered
under
the
microscope.
Real
viewfield®
Field
number/objective
mag¬
nification
•
Field
number
Generally,
it
means
the
diameter
of
the
field
diaphragm
of
the
eyepiece.
•
Depth
of
focus
Depth
(thickness)
of
specimen
image
appear¬
ing
sharp,
extending
above
and
below
the
focused
image
plane.

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