Nikon SC - Microscope Observation; Specimen Setup and Illumination; Focusing and Eyepiece Adjustment

III. OBSERVATION
1. Setting the specimen in position
1) Place the specimen on the stage holding in
position by means of the slide holder.
{Fig. 15)
2) When not using the mechanical stage, hold
the specimen securely with the two stage
clips. (Fig. 16)
2. Adjusting the illumination
1) Insert the power cord into the socket.
2) Raise the condenser to its upper limit, and
fully open the condenser diaphragm.
3) Turn the power switch & brightness control
dial to light the lamp, (Fig. 17)
Brightness is increased by turning toward the
thicker end of the arrow, and decreased to¬
ward the thinner.
4) Rotate the revolving nosepiece to swing the
4X or 10X objective into the optical path.
At this time, make sure that the objective
clicks properly into position,
3. Adjusting interpupillary distance (Fig. 18)
When using a binocular eyepiece tube, adjust
the interpupillary distance, so that both the
right and left viewfields become one.
4. Adjusting for eyesight difference (Fig. 19)
1) First, viewing into the right eyepiece with
the lOX objective in position, manipulate
the coarse and fine focus knobs to bring the
specimen into focus.
2} Then, viewing into the left eyepiece, turn
the diopter ring to bring the image into
sharp focus, without manipulating the focus
knobs.
0 index is put on the diopter ring.
Stage clips
Fig. 16
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