OM0133
13
7.5.3 CO
2
Regulators
T The regulator’s high-pressure stage direct from the supply cylinder must have a range of from 0
to 2000 PSI or 0 to 140 BAR. This gauge indicates actual tank pressure. The low-pressure stage
should have a range of 0 to 30 PSI or 0 to 2 BAR (100 PSI or 6 BARS maximum). This gauge
will indicate the actual CO
2
pressure to the Incubator. Some single stage CO
2
pressure regulators
have two gauges. USE A TWO-STAGE REGULATOR. All NuAire Incubators use CO
2
in such
small quantities that precise metering of CO
2
input pressure is important for maximum
performance.
To connect the regulator: First open the CO
2
cylinder slightly, for an instant (this is termed
“cracking the valve.”) This will blow out dust or dirt that may have collected in the valve outlet.
BE SURE to keep your face away from the valve outlet to protect your eyes from dust or dirt.
Second, MAKE SURE the regulator pressure-adjusting screw is released by turning it
counterclockwise until it turns freely. Third, attach the regulator to the cylinder valve and tighten
the connection nut with a wrench. BE SURE DISC SEAL IS IN PLACE BEFORE MAKING
CONNECTION.
7.5.4 CO
2
Connection
Connect the CO
2
supply from the low-stage of the two-stage regulator, to the inlet fitting located
on the Incubator back panel labeled “CO2 tank 1”. The filter should be inserted downstream of
the low-stage regulator before the inlet fitting on the Incubator as shown in the figure. Secure
each connection with the clamps supplied. Observe proper flow orientation of the filter (look for
“in” or a green dot on the filter). The tubing is easily cut with a sharp knife.
7.5.5 CO
2
Supply Adjustment
With the regulator OFF (i.e. fully counterclockwise), open the cylinder valve slowly usually 1 to
2 turns is sufficient.
NEVER STAND IN FRONT OR BEHIND THE REGULATOR WHEN OPENING THE
VALVE. ALWAYS STAND TO ONE SIDE.
The cylinder tank pressure should read 700 to 800 PSI or 48 to 55 BAR, more or less, depending
on the temperature of the cylinder. Next, turn the regulator’s pressure adjusting screw clockwise,
until the low-pressure gauge reads 20 PSI or 1.4 BAR. The CO
2
connection is now complete.
NOTE: OSHA requires the CO
2
tanks to be physically restrained (i.e. via chained to wall)
to prevent accidental damage to cylinder.
If optional feature Model Number I01, CO
2
Automatic Tank Switch (Internal) is purchased,
separate installation instructions are provided.
7.5.6 Checking the Connections
The connections can be checked for leakage by brushing a small amount of soapy water on each
one. Observe to see if any bubbles are blown. If not the connection is secured properly. If it is,
the cause of the leaking needs to be determined, (i.e. loose clamp or damaged hose) and
corrected.
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