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Olympus CH30 - Phase Contrast Attachments; Mounting and Using Phase Contrast

Olympus CH30
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©
Nomenciature
Light
Annuli
chansio
Cha.RS40
Faso
Contrast
Objectives
PCDATOX
PLPCOAGOXPL
Geniaring
Telescope
OTS]
<
@
Vounting
the
Phase
Contrast
Attachment
Mount
the
phase
annulus
(CH2-RS10/CH2-RS40)
in
the
same
way
as
the
filter
holder
+
Replace
previously
mounted
objectives
with
the
phase
contrast
objectives.
©
before
centration
Ator
centration
di
iii
Tum
the
revolving
nosepiece
to
move
the
objective
with
the
same
number
es
the
mounted
light
annulus.
Set
the
specimen
on
the
stage
and
ring
it
into
focus.
Remove
either
one
of
the
ογορίοσος
and
insert
the
centering
telescope
(CTI
into
the
eyepiece
tube
Rotate
the
CT's
upper
lens
assombly
so
that
the
bright
ing
©
light
annulus
inthe
condenser
and
the
dark
ring
(
(phase
annulus
in
the
objective)
come
into
focus.
(Fig.
31)
Rotate
the
two
ight
annulus
centering
knobs
@
unti
th
ght
annulus
and
phase
annulus
ae
concentric
and
superimposed.
(Figs.31
&
32)
Remove
the
CFS
and
insert
the
eyepiece
back
into
the
eyepiece
tube
to
perform
phase
contrast
observation.
To
increase
image
contrast,
mount
the
filter
on
the
filer
holder.

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