Camera settings
22
In this dialog box, check
whether correction
images of the Dark or
the Flatfield type, are
available.
Definition "Shad-
ing"
Every optical system with a camera and microscope creates, even when the devices
have been set up very carefully, image inhomogeneities in the illumination of the
sample. These are called shading.
The left illustration
shows an image where
the online shading
correction has not been
activated. In compar-
ison to the image on the
right, one can notice the
inhomogoneities in the
illumination.
For a shading correction, the shading will first be determined in order to be able to
make corrections to the acquired image. To determine which shading there is in the
image, two types of correction images will be acquired:
Dark correction
image
The Dark correction image is an acquisition during which no light falls on the camera.
Here, pixels with different brightness resulting from noise or different sensitivity of
individual pixels on the camera chip can be corrected by the correction image. The
Dark correction image is characteristic for each camera and needs only be acquired
once. It is valid for all objectives.
Flatfield correction
image
On the Flatfield correction image the illumination of the complete optical system with-
out a sample will be shown. An individual correction image must be made for every
objective and for every resolution of your camera.
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