9
Dichroic Mirrors and Filter Configurations of Fluorescence Mirror Units
Excitation
Method
Mirror Unit Dichroic Mirror Excitation Filter Barrier Filter Fluorochrome
U
U-MWU2
U-MNU2
DM400
BP330-385
BP360-370
BA420
· Autofluorescence observation
· DAPI staining: DNA
· Hoechest 33258, 33342 staining: Chro-
mosome
V U-MNV2 DM455 BP400-410 BA455
· Catechrolamine observation
· Serotonin observation
· Tetracyine staining: Bones, teeth
BV
U-MWBV2
U-MNBV2
DM455
BP400-440
BP420-440
BA475
· Quinacine & quinacine mustard stain-
ing: DNA
· Thioflavine S staining: Lymphocite
· Acriflavine staining: Nucleic acid
· CFP
U-MWB2 BP460-490
B U-MNB2 DM500 BP470-490 BA520IF
U-MSWB2 BP420-480
· FITC staining: Fluorescent antibody
· Acridine orange staining: DNA, RNA
· Auramine staining: Tubercle bacteria
· EGFP, S65T, RSGFP
IB
U-MWIB2
U-MNIB2
DM505
BP460-490
BP470-490
BA510IF
U-MWG2 BP510-550
G U-MNG2 DM570 BP530-550 BA590
U-MSWG2 BP480-550
· Rhodamine & TRITC staining: Fluores-
cent antibody
· Propidium iodide staining: DNA
· RFP
IG U-MWIG2 DM565 BP520-550 BA580IF
IY U-MWIY2 DM600 BP545-580 BA610IF Texas Red staining: Fluorescent antibody
Color Separation Filter Combinations
U U-MNUA2 DM400 BP360-370 BA420-460
For observing only the U-excitation stain,
when using U-excitation stain together
with FITC.
IB
U-MWIBA2
U-MNIBA2
DM505
BP460-490
BP470-550
BA510-550
For observing only the B-excitation stain,
when using B-excitation stain together
with FRITC.
Meaning of Mirror Unit Name
U- MN I B A 2
For color separation
Excitation (U, UV, BV, B, IB, G, IG or IY)
Bandwidth (SW: Superwide band. W: Wide band. N: Narrow band.)
Mirror unit
Universal
Model number
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