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Olympus BX3-URA - User Manual

Olympus BX3-URA
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INSTRUCTIONS
This instruction manual is for the Olympus Reflected Fluorescence System. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the use of this system, we
recommend that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
A X 7 8 8 8
BX3-URA
BX3-RFAS
U-LH100HGAPO
U-LH100HG
U-RFL-T
U-AN-2
BX3-25ND6
BX3-25ND25
REFLECTED
FLUORESCENCE
SYSTEM

Table of Contents

Questions and Answers

  • A
    Alex FloydJul 31, 2025
    Why can't I see anything through my Olympus BX3-URA Microscope, even though the burner is on?
    • J
      Jonathon StephensJul 31, 2025
      If the burner is on but the image from your Olympus Microscope is dark, first ensure the shutter is open. Then, check if the ND filter is engaged and remove it if necessary. Verify that the fluorescence mirror unit is correctly engaged and matches the specimen. Also, make sure that the aperture and field iris diaphragms are centered and fully enlarged.
  • K
    Kayla SantosAug 6, 2025
    What to do if the field of view on my Olympus Microscope is obscured?
    • R
      Rebecca JacksonAug 6, 2025
      If your Olympus Microscope's field of view is obscured, ensure the objective and fluorescence mirror unit are correctly engaged in the light path. The revolving nosepiece and ND slider should click into place properly. Also, check that the field iris diaphragm is not set too small; center the aperture iris diaphragm and field iris diaphragm, then fully enlarge the field iris diaphragm. Finally, ensure the mercury burner is properly centered.
  • A
    Angela SmithAug 11, 2025
    Why is the mercury burner flickering on my Olympus BX3-URA?
    • R
      Ricky SawyerAug 11, 2025
      If the mercury burner of your Olympus Microscope flickers or appears dark, wait at least 10 minutes after ignition for it to stabilize. If the issue persists and the hour counter indicates 300 hours (USH-103OL), replace the mercury burner. Also, ensure the burner is centered on the optical axis.
  • L
    Laura WebbAug 16, 2025
    How to improve the image quality of my Olympus BX3-URA?
    • C
      Caitlin JensenAug 16, 2025
      If the image from your Olympus Microscope appears low quality, not sharp, or poor in contrast, start by thoroughly cleaning the objective and filter for any dirt or dust. Also, verify that the fluorescence mirror unit matches the specimen you are viewing.
  • W
    watsonjohnAug 20, 2025
    What causes dark spots in the field of my Olympus Microscope?
    • A
      Amy PachecoAug 20, 2025
      If your Olympus Microscope field contains dark, spot-like areas, the burner or the burner side of the collector lens may be dirty or dusty. Clean these components to resolve the issue.
  • L
    Latasha HaysAug 25, 2025
    Why won't the mercury burner light on my Olympus Microscope?
    • K
      Katelyn SmithAug 26, 2025
      If the mercury burner on your Olympus Microscope does not light even when the main switch is set to “I” (ON), first, ensure all connectors are firmly connected. Then, check that the mercury burner is properly attached. Verify that the safety device in the lamp housing is correctly set up. If auto ignition is malfunctioning, set the main switch of the power supply unit to “ ” (OFF) then “I” (ON) again, repeating as needed.

Summary

Important Safety Information

Safe Use of the Equipment

Guidance on safe usage and precautions for the reflected fluorescence system.

Safety Precautions

General Safety Precautions

Essential safety measures to follow when operating the reflected fluorescence system.

Electrical Safety

Precautions related to electrical safety, grounding, and power supply unit handling.

Safety Symbols and Cautionary Notes

Safety Symbols and Cautionary Notes

Nomenclature of Components

Reflected Fluorescence Observation Procedure

Preparation Steps

Initial setup and preparation before commencing fluorescence observation.

Observation Steps

The actual process of observing specimens using the fluorescence system.

Using the Controls

Power Supply Unit Operation

Power Supply Unit Operation

Procedure for turning the power supply unit on and stabilizing the illumination.

Centering the Field Iris Diaphragm

Centering the Field Iris Diaphragm

Centering the Aperture Iris Diaphragm

Centering the Aperture Iris Diaphragm

Centering the Mercury Burner

Mercury Burner Centering with BX3-URA

Detailed steps for centering the mercury burner using the BX3-URA illuminator.

Precise Mirror Centering

Mounting Neutral Density (ND) Filters

Mounting Neutral Density (ND) Filters

Simultaneous Fluorescence Observations

Simultaneous Fluorescence and Phase Contrast

Simultaneous Fluorescence and Transmitted Light DIC

Troubleshooting Guide

Troubleshooting Guide

Common problems, their causes, and recommended solutions for the fluorescence system.

System Specifications

Assembly Diagram

Assembly Diagram

Assembly Procedures

Detailed Assembly Procedures

Attaching Mercury Burner and Lamp Housing

Attaching Mercury Burner and Lamp Housing

Step-by-step instructions for installing the mercury burner and lamp housing.

Power Supply Cord Selection

Olympus BX3-URA Specifications

General IconGeneral
ObjectivesPlanApo series
IlluminationLED
FocusingCoarse and fine focusing
Observation MethodsBrightfield, Darkfield, Phase contrast, DIC, Polarization, Fluorescence
Magnification Range1.25x to 100x (depending on objectives and observation tubes used)
Objective Turret6-position
Observation TubesBinocular, Trinocular
CondenserUniversal condenser

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