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Olympus BX3-RFAS - User Manual

Olympus BX3-RFAS
36 pages
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INSTRUCTIONS
This instruction manual is for the Olympus Reflected Fluorescence System. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the use of this system, we
recommend that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
A X 7 8 8 8
BX3-URA
BX3-RFAS
U-LH100HGAPO
U-LH100HG
U-RFL-T
U-AN-2
BX3-25ND6
BX3-25ND25
REFLECTED
FLUORESCENCE
SYSTEM

Questions and Answers:

Olympus BX3-RFAS Specifications

General IconGeneral
Observation MethodBrightfield, Darkfield, Phase contrast, DIC, Polarized light, Fluorescence
Microscope TypeUpright Research Microscope
IlluminationLED or Halogen
Nosepiece6-position revolving nosepiece
Optical SystemInfinity-corrected optical system
Objective LensesUIS2 series objectives
StageMotorized or manual stage
Camera PortC-mount camera port
Software CompatibilityOlympus cellSens software
DimensionsVaries by configuration
WeightVaries by configuration

Summary

Safety Precautions and Important Information

Important General Information

General information and guidance for safe operation of the system.

Nomenclature and Component Identification

Reflected Fluorescence Observation Procedure

Using the Controls and System Operation

General Precautions for Observation

Provides essential precautions for operating the reflected fluorescence system.

Selecting the Fluorescence Mirror Unit

Guidance on choosing the correct fluorescence mirror unit for specific applications.

Turning the Power Supply Unit On

Instructions for powering up the system and waiting for stabilization.

Centering the Field Iris Diaphragm

Procedure for aligning the field iris diaphragm for optimal illumination.

Centering the Aperture Iris Diaphragm (BX3-URA)

Procedure for aligning the aperture iris diaphragm for optimal contrast.

Centering the Mercury Burner

Steps for aligning the mercury burner for consistent illumination.

Mounting the ND Filters

Attaching the Light Shield Sheet

Simultaneous Fluorescence Observations

Simultaneous Reflected Fluorescence and Phase Contrast Observations

Combining reflected fluorescence with phase contrast observation methods.

Simultaneous Reflected Fluorescence and Transmitted Light DIC Observations

Combining reflected fluorescence with transmitted light DIC observation.

Troubleshooting Guide

Spectral Characteristics of Filters

Specifications and Technical Details

Assembly and Installation Procedures

Assembly Diagram

Visual guide showing the sequence of assembling microscope modules.

Detailed Assembly Procedures

Step-by-step instructions for assembling various microscope components.

Attaching the Mercury Burner and Lamp Housing

Procedure for correctly installing the mercury burner and lamp housing.

Making an Optional Fluorescence Mirror Unit

Instructions for fabricating custom fluorescence mirror units.

Lamp Housing Inspection Sheet

Proper Selection of Power Supply Cord

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