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Olympus BX3-25ND25 User Manual

Olympus BX3-25ND25
36 pages
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INSTRUCTIONS
This instruction manual is for the Olympus Reflected Fluorescence System. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the use of this system, we
recommend that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
A X 7 8 8 8
BX3-URA
BX3-RFAS
U-LH100HGAPO
U-LH100HG
U-RFL-T
U-AN-2
BX3-25ND6
BX3-25ND25
REFLECTED
FLUORESCENCE
SYSTEM

Questions and Answers:

Olympus BX3-25ND25 Specifications

General IconGeneral
Lens Barrel Diameter25 mm
Optical SystemUIS2
FocusingManual
IlluminationTransmitted Light
CorrectionPlan

Summary

Important Safety and Usage Information

Nomenclature

Reflected Fluorescence Observation Procedure

Using the Controls

General Precautions for Observation

General safety and operational guidelines for using the reflected fluorescence system.

Selecting the Fluorescence Mirror Unit

Guidance on choosing the correct fluorescence mirror unit based on excitation light.

Turning the Power Supply Unit On

Steps to safely power on the unit and allow it to stabilize.

Centering the Field Iris Diaphragm

Procedure to center the field iris diaphragm for optimal image contrast.

Centering the Aperture Iris Diaphragm

Procedure for centering the aperture iris diaphragm on BX3-URA illuminator.

Centering the Mercury Burner

Steps to align the mercury burner for proper illumination.

Precise Mirror Centering

Advanced procedure for precise adjustment of the mirror position.

Mounting the ND Filters

Instructions for inserting Neutral Density filters to control light intensity.

Attaching the Light Shield Sheet

How to attach the light shield sheet to prevent image degradation.

Simultaneous Fluorescence Observations

Simultaneous Fluorescence and Phase Contrast

Procedure for combining reflected fluorescence with phase contrast observation.

Fluorescence and Transmitted Light DIC

Procedure for combining reflected fluorescence with DIC observation.

Troubleshooting Guide

Spectral Characteristics of Filters

Specifications

Assembly

Assembly Diagram

Diagram illustrating the sequence of assembling various microscope modules.

Detailed Assembly Procedures

Step-by-step instructions for assembling the microscope components.

Attaching Mercury Burner and Lamp Housing

Instructions for attaching the mercury burner and lamp housing.

Making Optional Fluorescence Mirror Units

Guide to fabricating custom fluorescence mirror units.

Lamp Housing Inspection Sheet

Proper Power Supply Cord Selection

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