6
REFLECTED FLUORESCENCE OBSERVATION
PROCEDURE
}For the operating procedures for simultaneous observations of phase contrast or transmitted Nomarski DIC observation
with fluorescence observation, see chapter 4, “SIMULTANEOUS FLUORESCENCE OBSERVATION METHODS” (page 15).
(Controls Used)
(Page)
Preparation
· Attach the fluorescence mirror units and objectives which are suitable for the observation method to be used. (P. 8 - 10)
· Center the mercury burner. (P. 13 - P. 14)
· Set the main switch @ of the BX-UCB control box.
Set the main switch to “ I ” (ON) and wait until
the arc image stabilizes (5 to 10 minutes).
² Main switch (P. 10)
Place the specimen on the stage.
³ Specimen holder
| X/Y-axis knobs
Engage the mirror unit matching the speci-
men.
ƒ MU+ (Mirror Unit Right) or
MU- (Mirror Unit Left) button
Engage the objective in the light path and bring
the specimen in focus.
… Objective button
† RSHT (shutter) button
‡ Stage UP/DOWN button
Engage a ND filter in the light path
as required.
Š ND filter (P. 14/23)
Adjust the collector lens so that the field of
view is uniform and brightest.
‰ Collector lens focusing knob (P. 13)
Adjust the field iris diaphragm. ‹ Field iris diaphragm knob (P. 11)
Adjust the aperture iris diaphragm.
ΠAperture iris diaphragm knob (P. 12)
Start observation.
}Engage the shutter if you interrupt observation for a short time. † RSHT (shutter) button
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