Olympus U-LH100HG - 2 REFLECTED FLUORESCENCE OBSERVATION PROCEDURE

6
(Controls Used)
(Page)
Start observation.
(P. 11)
Set the main switch to “ I ” (ON) and wait for
the arc to stabilize.
REFLECTED FLUORESCENCE OBSERVATION PROCEDURE
}If you need simultaneous observation of reflected fluorescence observation with the phase contrast observation or trans-
mitted light Nomarski Differential Interference Contrast (DIC) observation, please read Chapter 4, “SIMULTANEOUS FLUO-
RESCENCE OBSERVATION”. (Page 16)
Preparation
· Attach the fluorescence mirror unit and objective matching the observation method. (Pages 8 to 11)
· Center the mercury burner. (Page 14 or 15)
@ Main switch
Place the specimen on the stage.
² Specimen holder
³ X-/Y-axis knobs
Engage the fluorescence mirror unit
matching the specimen in the light path.
| Mirror unit turret
Engage the objective in the light path and
focus on the specimen.
ƒ Revolving nosepiece
… Coarse/fine adjustment knobs
Engage an ND filter in the light
path as required.
Adjust so that the entire field is
uniform and brightest.
† ND filters
‡ Collector lens focusing knob
Adjust the field iris diaphragm.
Š Field iris diaphragm knob
Adjust the aperture iris diaphragm.
‰ Aperture iris diaphragm knob
}Engage the shutter if you interrupt observation for a short time.
‹ Shutter knob
(P. 15)
(P. 14)
(P. 12)
(P. 13)
(P. 12)