Olympus U-LH100HG - Page 13

9
Dichroic Mirror and Filter Configurations of Fluorescence Mirror Units
Excitation
Method
Mirror Unit Dichroic Mirror Excitation Filter Barrier Filter Fluorochromes
· Autofluorescence observation
· DAPI: DNA staining
· Hoechest 33258, 33342: Chromosome
· Catecholamine
· Serotonin
· Tetracyline: Bones, teeth
· Quinacrine, quinacrine mustard:
Chromosome
· Thioflavine S: Lymphocyte
· Acriflavine: Nucleic acid
· ECFP
· FITC: Fluorescent antibody
· Acridine orange: DNA, RNA
· Auramine: Tubercle bacillus
· EGFP, S65T, RSGFP
· Rhodamine, TRITC: Florescent antibody
· Propidium iodide: DNA
· RFP
Texas Red: Fluorescent antibody
Color Separation Filter Combinations
For observing only the U-excitation stain,
when using U-excitation stain together
with FITC.
For observing only the B-excitation stain,
when using B-excitation stain with TRITC
or Texas Red.
Mirror Unit Name Meaning
For color separation
U- MN I B A 2
Excitation (U, V, BV, B, IB, G, IG or IY)
Bandwidth (SW: Superwide band. W: Wide band. N: Narrow band.)
Mirror unit
Universal
U
V
BV
B
IB
G
IG
IY
U U-MNUA2
IB
U-MWIBA3
U-MNIBA3
DM400 BP360-370 BA420-460
DM505
BP460-495
BA510-550
BP470-495
U-MWU2
DM400
BP330-385
BA420
U-MNV2
DM455 BP400-410 BA455
U-MWBV2
DM455
BP400-440
BA475
U-MWB2
U-MNB2
U-MSWB2
DM500
BP460-490
BP470-490
BP420-480
BA520IF
U-MWIB3
U-MNIB3
DM505
BP460-495
BP470-495
BA510IF
U-MWG2
U-MNG2
U-MSWG2
DM570
BP510-550
BP530-550
BP480-550
BA590
U-MWIG3 DM570 BP530-550 BA575IF
U-MWIY2 DM600
BP545-580 BA610IF
U-MNU2
BP360-370
U-MNBV2 BP420-440
Model number (2 or 3)
For observing only the G-excitation stain,
when using G-excitation stain together
with Cy5.
G
U-MWIGA3
U-MNIGA3
DM570
BP530-550
BA575-625
BP540-550