GFPIII operation manual Opti-Sciences, Inc.
21
Data formats:
The format is stored in a comma separated variable format that can be easily imported into popular
spreadsheet programs. A header in each file identifies the specific data field.
Sample Test Format:
Sample #, Date, Time, Raw Signal, CFR, Chlorophyll content in mg m
-2
1,Oct/11/2011,11:49:43,751,0.33, 600
2,Oct/11/2011,11:50:02,796,0.35, 613
Specifications:
• Source:
Solid state modulated LED device providing excitation wavelengths from 460 nm with a half
bandwidth of 15 nm
• Detector:
Two solid state detectors . One filtered for detection from 698nm to 708 nm and the other
filtered for a range from 730nm to 740nm.
• Noise:
Typically < 2% of full scale, but is dependant upon calibrated range
• Sensitivity:
signal integration over 5 seconds.
• Sensing probe:
Fiber has 0.10” diameter active region contained within a 0.15" diameter stainless steel tip
• Calibration:
Solid state sensors and light sources provide no significant variation over the life of the
instrument. The measurement represents a ratio of fluorescence at two different emission
wavelengths. The research shows that this ratio provides a precise measurement of Chlorophyll
content from 41mg m-2 to 675 mg m-2 using Beech, Elm or Wild Vine. Calibration may be
checked by comparing chemical tests to read-out values. Parameters in the direct chlorophyll
content formula may be changed to conform with chemical test results for any unexpected non-
conforming species. The values and equation in the instrument conform with the equation
supplied by Gitleson (1999).
• Repeatability is dependent on the emission signal strength. Measurements in the yellow signal
strength range may require multiple measurements and averaging for more reliable results.
• User Interface:
320x480 color TFT LCD screen with touch panel surface for user input
• Data capacity:
Internal, non volatile 2GB memory for storage of calibration and data files
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