GFPIII operation manual Opti-Sciences, Inc.
The Kautsky induction effect
The CCM-300 has been designed to minimize this variable. The graphs below show that whether a
sample is dark adapted for 20 minutes, or light adapted for 20 minutes, measurements are not significantly
affected by the Kautsky induction effect. This is not true of other fluorescence ratios and methods as
described by Bushman (2007). Measurements were made immediately after dark and light adaptation, and
again every 15 seconds for 20 minutes.
With the CCM-300 using the F735 nm / F700 nm ratio the Kautsky induction effect is negligible.
Negligible Kautsky induction effects with the CCM-300
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Unlike the CCM-300 F735 / F700 fluorescence ratio, other ratios such as F 690/ F735 have shown
Kautsky induction effects. It has been found that the fluorescence emission ratio 690/ 735 is affected by
the onset of photosynthesis and exposure to light (The Kautsky effect). Bushman (2007) shows that the
shorter wavelength fluorescence emission spectra changes faster, during the Kautsky induction, than the
longer wavelength emission spectra. The affects are also minimized by using a very low intensity
modulated light source.
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