6 Lock the membrane filter in place by pushing
the filter funnel firmly into position in the blue
rubber base. Take care to not touch the internal
surface of the filter funnel with your hand.
7 Ensure the filter funnel is aligned correctly in the
‘Filtration’ position, indicated by the graphic
on the side of the filter funnel.
8 Pour the water sample into the filter funnel
up to the 100ml graduation (or less if using
a smaller sample).
9
Discard the excess water from the sampling
cup
then insert the filter funnel/base assembly
into position in the sampling cup. Care is needed
to prevent sample spillage from the filter funnel
when assembling.
NOTE: If the sample is not to be collected in the
sampling cup part of the membrane filtration
unit, then the filter funnel/base assembly can
be inserted into position in the sampling cup
straight away.
10 Connect the hand vacuum pump and
silicone tubing to the MFU.
11 Use the pump to create a vacuum and
commence the filtration. The sample level
in the filter funnel will fall rapidly.
Do not pump too many times so as to avoid
drawing excess air through the membrane filter.
The water passes through the pores in the
membrane filter and is collected in the sampling
cup. Any bacteria in the sample are collected
on the surface of the membrane filter.
17
5
Sample Processing - Membrane Filtration