palintest Wagtech Potalab+ (M) User Manual

5
Sample Processing - Membrane Filtration
18
12 When all of the sample has been filtered,
detach the vacuum pump and remove the
filter funnel from the rubber base. The
membrane filter is now ready to be removed
and placed in the petri dish containing the
absorbent pad and growth media.
13 Remove one of the sterile petri dishes
previously
prepared from the rack and place onto the
work-surface. (Prepared petri dishes should
contain an absorbent pad,
if not, follow the
procedure in Section 3.3).
14 Taking care to only handle the petri dish by
the sides, remove the lid and place it on the
work surface.
15 Take an MMD containing liquid MLSB media.
Shake well then remove the blue screw lid and
place it, lid-down, onto the work-surface.
16 Lift the petri dish containing the absorbent pad
and hold between thumb and forefinger.
17 Take the MMD containing the liquid MLSB and
carefully pour the media onto the absorbent
pad in the petri dish in a single, decisive
pouring motion. Always ensure that the petri
dish is raised from the surface when pouring
the media. Never use the plastic Pasteur
pipettes to dispense media onto the pads.
18 Ensure the pad is well saturated with a small
excess of MLSB visible at the edges. This should
equate to between 2.5 and 3.0mls. If too
much media is dispensed, simply pour the
excess away. Place the petri dish back down
on the work surface.

palintest Wagtech Potalab+ (M) Specifications

General

Brand	palintest
Model	Wagtech Potalab+ (M)
Category	Measuring Instruments
Language	English