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Wallac 1420
PerkinElmer Wallac 1420 User Manual
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1420-922-07
March 2003
User manual
1420
Software version 3.00
PerkinElmer Life and Analytical Sciences, Wa
llac Oy, P.O. Box
10,
FIN-20101 Turku, Finland.
Tel: 358-2-2678111. Fax:
358-2-2678357. Website:
www.per
kin
elm
er.
com
/li
fes
cie
nc
es
2
Table of Contents
Table of Contents
3
Introduction
11
System Overview
13
Enhanced Security Mode
14
Software Installation
15
Operation Overview
16
Operating Wallac 1420
17
Starting up Wallac 1420
19
Without Enhanced Security Mode
19
With Enhanced Security Mode
19
Instrument Control
20
Live Display
21
Temperature
23
Barcode Options
24
Plate Orientation
24
Starting Operation with the Start Button
25
Starting Operation with the Start Wizard
28
Starting Operation and Using a Protocol Number Barcode
30
Stacker Operation
31
Starting Operation, no Protocol Number Barcode
31
Starting Operation and Using a Protocol Number Barcode
33
Ending Operation
34
Stop Button
34
End Button
34
Button on Wallac 1420
35
Light on the Button
35
Protocol Editing
37
Explorer
39
Explorer Folders
40
Explorer Icons
40
Protocol Selection
41
Protocol Editor
42
Samples
43
Measurement
46
Measurement Mode
46
Measurement Operations
47
Down Button
47
Up Button
47
Delete Button
47
Dispense Button
47
Delay Button
48
Shake Button
48
Label Button
48
Kinetics Button
49
Scanning Button
49
Injector Position During Measurement
49
Plate
50
Plate Repeat Parameters
50
Measurement Height
50
Plate Type
51
Temperature Level Checking
51
Outputs
52
Events
54
Keywords
55
General
56
File Menu
57
Save
57
Print
57
Print Preview
57
Print Setup
57
Start
57
Exit
57
Tools Menu
58
Result Viewing
59
Latest Results
61
Results in the Explorer
61
List
63
Plate Map
64
Protocol
65
Notes
66
Error
66
Print
66
Export Results
66
Tools Menu
67
Tools Menu
69
User Level
70
Labels
71
Time-Resolved Fluorometry
73
Protocol Name
73
Flash Energy Area
74
Flash Energy Level
74
Excitation Filter
74
Light Integration Capacitors
74
Note
75
Light Integration Reference Level
75
Emission Filters
75
Emission Aperture
75
Counting Parameters
76
Factory-Set Values for the Main Counting Window
76
Beam Size
76
Second Measurement Parameters
76
Fluorometry
77
Protocol Name
77
CW-Lamp Energy
78
CW-Lamp Control Mode
78
CW-Lamp Filters
78
Excitation Aperture (Wallac 1420-040/041/042/043 Only)
78
Emission Filters
78
Emission Aperture
79
Counter Position
79
Counting Time
79
Second Measurement Parameters
79
Photometry
80
Protocol Name
80
Absorbance Mode
80
Flash Lamp Filter
80
CW-Lamp Filters
81
Excitation Aperture (Wallac 1420-040/041 Only)
81
Reading Time
81
Second Measurement
81
Luminometry
82
Protocol Name
82
Emission Filters
82
Emission Aperture
83
Counting Time
83
Second Measurement
83
Fluorescence Polarization (Wallac 1420-040/041/042/043 Only)
84
Protocol Name
84
CW-Lamp Energy
85
CW-Lamp Filter
85
Polarizer Aperture
85
Emission Filter
85
Emission Aperture
85
Counting Time
85
G Factor
86
Lance
87
Protocol Name
87
Flash Energy Area
88
Flash Energy Level
88
Excitation Filter
88
Light Integration Capacitors
88
Note
89
Light Integration Reference Level
89
Emission Filter
89
Emission Aperture
89
Counting Parameters
90
Factory-Set Values for the Main Counting Window
90
Label
90
Delay 1
90
Delay 2
90
Window 1
90
Window 2
90
Cycle
90
Beam Size
90
Second Measurement Parameters
90
LANCE Label Properties
91
Filters
92
Emission Filters
93
CW-Lamp Filters
94
Filter Slides
95
Emission Filter Slide
96
Physically Changing the Emission Filter Slide
97
CW-Lamp Filter Wheel
98
Physically Changing the CW-Lamp Filter Wheel
99
Changing the CW-Lamp
99
Changing Filters in the Software
100
Filter Properties
101
Eusm Dual Label Normalization
102
Plate Type Selection
102
Preparing the Plate
103
Measurement Status Page
104
Measurement Warning Page
104
Normalization Confirmation Page
105
LANCE Normalization
106
Using the LANCE Normalization Wizard
106
Protocol Selection
107
Preparing the Plate
108
Measurement Status Page
109
Measurement Warning Page
110
Normalization Confirmation Page
111
Using LANCE Normalization Samples in the Assay
112
Plate Dimension Wizard
114
Plate Selection
115
Technology Selection
115
Preparing the Plate
116
Define the Centre Point of the Corner Wells
117
Miscellaneous Settings
119
Plate Type
121
Plate Name
121
Number of Well Rows
122
Number of Well Columns
122
Height of the Plate
122
Strip Orientation
122
Plate Dimensions
122
Tune
122
Well Names
122
Instrument Properties
122
Options
123
General
123
Instrument Hardware
126
Measurement Technologies
127
Exiting
129
File Menu
131
Automatic Logout
131
Troubleshooting
133
Out of Memory
135
Display Problems
136
Performance Degradation
136
Processing Error
137
System Requirements Not Met
137
Appendices
139
Appendix 1: Connecting Multicalc to Wallac 1420
141
Introduction
141
Wallac 1420 Multicalc Assay Protocols
141
Installation of Wallac 1420 Output Option in Multicalc
142
Multicalc Assay Protocols
142
Usage of Multicalc
142
Appendix 2: Plate Types for Stackers
143
Wallac
143
Nunc
143
Costar
143
Greiner
143
Appendix 3: Dispenser Operation
145
Introduction
145
Emptying the Waste Vial
146
Preparing the Tubing
148
Dispenser Maintenance
149
Measurement
152
Maintenance
153
Needle Maintenance
153
Appendix 4: Enhanced Security Special Functions
155
User Groups and Permissions
155
User Management
156
Password Control
158
Audit Trail
160
Archive
162
Security Settings
163
Index
167
4
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PerkinElmer Wallac 1420 Specifications
General
Brand
PerkinElmer
Model
Wallac 1420
Category
Laboratory Equipment
Language
English
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