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PerkinElmer Wallac 1420 User Manual

PerkinElmer Wallac 1420
171 pages
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1420-922-07
March 2003
User manual
1420
Software version 3.00
PerkinElmer Life and Analytical Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland.
Tel: 358-2-2678111. Fax: 358-2-2678357. Website: www.perkinelmer.com/lifesciences

Table of Contents

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PerkinElmer Wallac 1420 Specifications

General IconGeneral
BrandPerkinElmer
ModelWallac 1420
CategoryLaboratory Equipment
LanguageEnglish

Summary

Introduction to Wallac 1420

Enhanced Security Mode

Details the Enhanced Security mode for 21 CFR Part 11 compliance, access control, data security, and audit trails.

Software Installation

Provides step-by-step instructions for installing the Wallac 1420 software from a CD.

Operating the Wallac 1420

Starting Up Wallac 1420

Describes the initial startup procedure for Wallac 1420, with and without enhanced security.

Instrument Control

Details the Instrument Control tab for starting, stopping, and ending measurements.

Starting Operation with the Start Button

Provides instructions on starting measurements using the Start button.

Starting Operation with the Start Wizard

Explains how to use the Start Wizard for defining plates and sample positions.

Starting Operation with a Protocol Number Barcode

Details how to start measurements using a protocol number barcode.

Stacker Operation

Describes how to operate the instrument with stackers, including loading and starting.

Protocol Editing

Protocol Selection

Details how to open, copy, paste, start, or create protocols.

Protocol Editor

Outlines the seven tabs within the Protocol Editor for setting parameters.

Measurement Tab

Specifies the measurement sequence to be used in a protocol.

Measurement Operations

Lists operations like Dispense, Delay, Shake, Label, and their parameters.

Dispense Button

Details parameters for a dispense operation: injectors, volume, speed, increment, replicates, injection mode.

Kinetics Button

Details parameters for kinetic measurements: repeats, delay, and label selection.

Scanning Button

Explains parameters for scan measurements: steps, displacement, and distribution.

Outputs Tab

Describes selecting output devices (printer/file) and output formats.

Result Viewing

Exporting Results

Explains how to export results to files like Excel, Tab delimited text, or MultiCalc.

Tools Menu Functions

Tools Menu Overview

Lists operations available in the Tools menu, affecting the whole system.

Time-Resolved Fluorometry Parameters

Covers parameters for time-resolved fluorometry, including flash energy and filters.

Fluorometry Settings

Explains fluorometry, light selection, and parameters like protocol name.

Photometry Settings

Explains photometry, reference measurement, and absorbance calculation.

Fluorescence Polarization Parameters

Explains fluorescence polarization, light polarization, and calculation of mP.

G Factor Calculation

Explains the G-factor calculation and its use in correcting polarization values.

LANCE Homogeneous Time-Resolved Fluorescence

Introduces LANCE time-resolved fluorescence and its parameters.

LANCE Label Properties

Details specific LANCE labels and their properties for different assays.

EuSm Dual Label Normalization

Details the procedure for EuSm Dual label normalization using a wizard.

LANCE Normalization Wizard

Details LANCE normalization using a wizard to correct for various effects.

Plate Dimension Wizard

Explains using the wizard to fine-tune plate dimensions for exact positioning.

Options Settings

Covers General, Instrument hardware, Measurement technologies, and Automatic logout tabs.

Instrument Hardware Options

Shows installed instrument hardware options like Stacker, Barcode reader, and Temperature control.

Exiting the Software

Troubleshooting Common Issues

Out of Memory Errors

Addresses 'Out of memory' warnings, virtual memory settings, and disk space.

Processing Errors

Explains dialogues for Instrument server errors or communication issues.

System Requirements Not Met

Details dialogues for unmet system requirements during Manager startup.

Appendix 1: Connecting MultiCalc to Wallac 1420

Installation of Output Option in MultiCalc

Covers copying files and setting properties for Wallac 1420 output in MultiCalc.

Appendix 2: Plate Types for Stackers

Appendix 3: Dispenser Operation

Dispenser Maintenance

Details common dispenser maintenance operations like Fill, Empty, and Flush.

Post-Measurement Maintenance

Covers post-measurement maintenance: Empty command and tubing flush.

Appendix 4: Enhanced Security Special Functions

User Groups and Permissions

Explains user groups (Admin, Service, Auditor, Editor, Operator) and their permissions.

User Management

Details adding, removing, and editing users and managing password control.

Password Control Settings

Allows setting maximum/minimum password age, length, and uniqueness.

Account Lockout Settings

Details setting lockout properties for failed login attempts.

Audit Trail Functionality

Allows viewing operations performed by authorized users.

Archiving Audit Trail Entries

Details archiving audit trail entries and deleting archived entries from the database.

Archiving Results

Describes archiving results and protocol parameters as text files.

Security Settings Configuration

Allows changing the application account password via the Security Settings dialogue.

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