Pickering Laboratories PCX 5200 - Page 4

PCX 5200 User’s Manual Contents
Chapter 3 • Installation
Unpacking........................................................................................................... 3-1
Site Requirements .............................................................................................. 3-1
HPLC System Requirements ...................................................................................................3-1
Space Requirements................................................................................................................3-2
Electrical Requirement .............................................................................................................3-2
Inert Gas...................................................................................................................................3-2
Miscellaneous Supplies............................................................................................................ 3-2
Installation of the PCX5200 ................................................................................ 3-2
Layout of the HPLC and PCX5200 ..........................................................................................3-2
Note About Fittings ...................................................................................................................3-3
Inert Gas...................................................................................................................................3-3
Reagent Reservoir Connections ..............................................................................................3-3
Pressure-interlock Connections ...............................................................................................3-4
Injector Connections ................................................................................................................3-6
Detector Connections...............................................................................................................3-6
Guard & Analytical Column Installation....................................................................................3-7
Reagent Pump .........................................................................................................................3-8
Piston-wash System.................................................................................................................3-8
Priming the Reagent Pump ...................................................................................................... 3-9
System Testing & Verification...................................................................................................3-9
Shutdown.......................................................................................................... 3-10
Short-term ..............................................................................................................................3-10
Medium-term .......................................................................................................................... 3-11
Long-term ...............................................................................................................................3-12
Chapter 4 • Routine Maintenance & Troubleshooting
Initial System Testing .......................................................................................... 4-1
Test Chromatogram.................................................................................................................. 4-1
Paramater Log ......................................................................................................................... 4-1
Precautions & Problem Prevention..................................................................... 4-2
Mobile Phase ........................................................................................................................... 4-2
Back-flow Prevention ............................................................................................................... 4-2
Reactor Precautions.................................................................................................................4-2
Electrical Precaution ................................................................................................................ 4-2
Routine Maintenance.......................................................................................... 4-3
Reagent Pump .........................................................................................................................4-3
Pump Seal Replacement ......................................................................................................... 4-3
Check-valves............................................................................................................................4-6
Cleaning Check-valves ............................................................................................................ 4-6
Removing Check-valves .......................................................................................................... 4-7
Installing New Check-valves ....................................................................................................4-7
Rebuilding Check-valves .........................................................................................................4-8
Pre-column Filter ......................................................................................................................4-9
Reagent Filter.........................................................................................................................4-10
Ambient Reactor (not applicable to single reagent PCX5200) .............................................. 4-11