PCX5200 User’s Manual Chapter 3
3-12
1. Replace the reagent(s) with water and draw 10 mL through the prime/purge valve.
2. Set the HPLC pump at the normal flow rate. Choose an eluant that elutes contaminants
from the column. Turn on the PCX5200 to flush the system for 15 minutes. Set the
reactor temperature to < 60˚C.
3. Turn the reagent pump on and flush the entire system for 10 minutes.
4. Depressurize the system by disconnecting the “To Detector” fitting. Disconnect the outlet
of the column and remove the column and guard. Plug them tightly.
5. Replace the column and guard with a restrictor or small ID tubing and unions so there
are no open lines.
6. Remove any buffers from the HPLC and replace them with water.
7. Increase the flow rate of the HPLC to give 500 psi on the post-column system.
8. Flush the entire system for 30 minutes, then replace the water on the HPLC with 80/20
water/methanol.
9. Replace the water in the reagent reservoir with water / methanol (80/20).
10. Start the HPLC and reagents pumps, and flush the system for another 10 minutes.
11. Relieve the pressure in the reagent gauge(s) by briefly opening the bypass valve(s).
12. Let the system drain for 1–2 minutes.
13. Loosen the fitting at the inlet of the 100 psi external back-pressure regulator, relieving
pressure on the post-column system. Place paper towels under the back-pressure
regulator to absorb any escaping liquid.
14. Turn off the gas at the toggle valve of the gas manifold assembly and vent the
reservoirs.
15. Turn off the main power of the PCX5200.
16. Turn off the HPLC system.
17. Turn off the inert gas source.
Long-term
(7 days or longer)
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