PCX5200 User’s Manual Chapter 3
3-7
1. A set of PEEK tubing is provided in the Application Kit for installing your Pickering
column. Select the column that you wish to install, and the corresponding tubing set.
Refer to Figure 3-3 for column placement and door operation.
2. Open the column oven door. Be careful not to knock off the reagent bottle(s) if they are
placed on top of the PCX5200.
3. LOOSELY Connect the outlet of the guard column to the inlet of the analytical column.
4. Loosely fit the inlet of the guard column to the eluant heat exchanger. Carefully lay the
analytical column into its slot in the heating block.
5. Turn on the HPLC pump at 0.2 ml/min at 100% of the column storage solution.
6. Wait for liquid to drip from the heat exchanger. Tighten the connection to the guard. Wait
for liquid to drip from the inlet of the column and then tighten the connections.
7. A loose end of tubing with Fingertight™ fitting in the column oven leads to the first
mixing tee. Attach the loose end to the outlet of the analytical column.
8. Close the column oven door.
Guard & Analytical
Column Installation
Figure 3-3
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