PCX5200 User’s Manual Chapter 3
3-8
The reagent pumps have been calibrated to 0.30 mL/min at the factory and should not
need further adjustment.
Important! Before starting the post-column pump, connect a syringe and flush the
piston-wash system with 80:20 water:methanol (4 mL each time, at least twice a day).
Alternatively, you may connect it in series with the piston seal-wash system on your
HPLC so that you can flush them at the same time. The piston-wash system is designed
to flush the back-end of the primary seal which significantly extends seal life. However,
if a pump with a piston-wash system is used without liquid in the piston-wash system,
the secondary seal will wear out quickly (because it is dry). It can then scratch the
piston and the scratched piston will in turn cause the primary seal to fail.
Reagent Pump
Piston-wash System
Syringe and Luer adapter
Waste
Syringe and Luer adapter
Waste
Figure 3-4
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