PCX5200 User’s Manual Chapter 3
3-10
Important! If the system will not be used immediately after the installation, the
system must be shut down properly. Generic shutdown procedures are given below but
we advise you to follow the specific shutdown procedures in the Application Manual.
Upon completion of the analyses, use one of the following two procedures to shut down
the PCX5200 system properly. These procedures can prevent potential column damage,
reaction coil blockage, high background fluorescence, reagent precipitation, or other
problems.
1. Turn off the PCX5200 either manually by pressing the ENABLE key or via the
“Slowdown” program (see below).
2. Set the HPLC pump at the normal flow rate to flush the system for at least 20
minutes to allow the heated reactor to cool. Choose an eluant that elutes
contaminants from the column; for example, methanol for a reversed-phase column
and regenerant for an ion-exchange column.
3. Set the HPLC pump to ≤ 0.1 mL/min.
4. Turn off the detector lamp.
5. You may also program a slowdown method to accomplish all the above steps.
Step Time (min) % Eluant Flow (mL/min)
0 0 100 0.02
1 5 100 0.02
2 5.1 100 Normal flow*
3 25 100 Normal flow*
4 25.1 100 0.02
(*) Normal flow rates are different for various columns. Follow the instructions that
came with your column.
Shutdown
Short-term
(up to 3 days)
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