8
procedure will empty the tip(s) and ensure
accurate delivery.
4. Release the operating button to the starting
position. If necessary change the tip(s) and
continue pipetting.
7.2. Reverse pipetting
A selected volume plus an excess is aspirated
into the tip. The delivery is done without blow-
out, and so the excess volume remains in the tip.
The reverse technique is recommended for high
viscosity, biological or foaming liquids, or very
small volumes of liquid.
1. Depress the operating button all the way to
the second stop. Place the tip(s) just under the
surface of the liquid (2-3 mm) and smoothly
release the operating button up to the
starting position.
2. Withdraw the tip(s) from the liquid touching
against the edge of the container to remove
excess.
3. Deliver the preset volume by smoothly
depressing the operating button to the first
stop. Hold the operating button at the first
stop. The liquid that remains in the tip(s)
should not be included in the delivery.
4. The remaining liquid should now be discarded
with the tip(s) or delivered back into the
container vessel.
NOTE: If reverse pipetting technique is used the
pipette might need recalibration.
8. Storage
When not in use it is recommended that your
Proline pipette is stored in a vertical position. See
Installing the pipette holder (Chapter 4).
9. Testing the
performance and
recalibration
It is recommended to check the performance of
your pipettes regularly (e.g. every 3 months) and
always after in-house maintenance. However,
the user should establish a regular testing
routine for their pipettes with regard to accuracy
First stop
Second stop
To Next Page
Loading...
Need help?
General
