7
5. Release the tip ejector and test that it runs
smoothly.
6.4. Protective filters
The new tip cones of Sartorius pipettes (>10
µl) allow the use of a exchangeable filter as an
option. The filter prevents liquids and aerosols
from entering the pipette. Two types of filters are
available: a Safe-Cone Filter Standard and a Safe-
Cone Filter Plus (see cat.no. in Chapter 2.1). Plus
filters block the passage of liquids completely. The
filters do not affect the calibration of the pipette.
NOTE: Change the filter on the tip cone regularly
(after 50-250 pipettings).
7. Pipetting techniques
1. Make sure that the pipette, tips and the liquid
are at the same temperature.
2. Make sure that the tip is firmly attached to
the tip cone.
3. Hold the pipette vertically when aspirating
the liquid and place the tip only a few
millimetres into the liquid.
4. Prerinse the tip before aspirating the liquid
by filling and emptying the tip 5 times. This is
important especially when dispensing liquids
which have a viscosity and density different
from water.
5. Always control the operating button
movements with the thumb to ensure
consistency.
7.1. Forward pipetting
This technique employs the blow-out function
ensuring complete delivery of the liquid.
1. Depress the operating button to the first stop.
2. Place the tip(s) just under the surface of the
liquid (2-3 mm) and smoothly release the
operating button up to the starting position.
Carefully withdraw the tip from the liquid,
touching against the edge of the container
to remove excess.
3. Liquid is dispensed by gently depressing the
operating button to the first stop. After a
short delay continue to depress the operating
button to the second stop (= blow-out). This
Starting position
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