6
6. Operating the pipette
6.1. Volume setting
The volume of the pipette is clearly shown through
the handle display window. The delivery volume
(variable volume pipettes only) is set by turning
the operating button clockwise or anticlockwise.
When setting the volume, please make sure that:
1. The desired delivery volume clicks into place.
2. The digits are completely visible in the display
window.
3. The selected volume is within the pipette’s
specified range.
Do not turn the operating button outside the
volume range because it may jam the mechanism
and damage the pipette.
6.2. Sealing and ejecting tips
Sartorius tips are recommended for use with
Proline pipettes. Before fitting a tip ensure that
the pipette tip cone is clean. Press the tip on the
cone of the pipette firmly to ensure an airtight
seal. The seal is tight when a visible sealing ring
forms between the tip and the black tip cone.
Each pipette is fitted with a tip ejector to help
eliminate the safety hazards associated with
contamination. The tip ejector needs to be pressed
firmly downwards to ensure proper tip ejection.
Make sure that the tip is disposed of into a
suitable waste container.
6.3. Two-in-one pipettes
The Proline fixed volume pipettes 5 and 10 µl, and
variable volume pipettes 0.5-10 µl can be used
with both 10 µl and 200 µl tips. The pipettes are
supplied with two ejector collars and are factory
fitted with the ejector suitable for 10 µl tips.
When using 200 µl tips, please change the ejector
collar by following the instructions below:
1. Push the tip ejector down (1.).
2. Push the opening tool pin between the
ejector bar and ejector collar to release the
locking mechanism (2.).
3. Pull the ejector collar off (3.).
4. Install the ejector collar by pushing the collar
locking pin into the ejector bar hole, keeping
the tip ejector pushed down.
1.
2.
3.