SYSMEX KX-21 - Manual Calibration

CALIBRATION
Sysmex KX-21 Operator’s Manual -- Revised October 1998 6-9
3. MANUAL CALIBRATION
In manual calibration, calculated calibration value can be entered using the numeric keys.
3.1 Calculating Calibration Value
(1) Analyze more than five samples, each three times, by the reference method and average
HGB and HCT values.
(2) Gently mix the same samples sufficiently and analyze them in the whole blood mode.
(3) When there is a difference between data gained by analysis with this instrument and the
reference value gained by the reference method, use the following formula to calculate
the new calibration value:
= ×
[Example]
Average of HGB values gained by the reference method = 15.6 g/dL
Average of HGB values gained by this instrument = 15.5 g/dL
Previous calibration value of HGB = 100.0%
100.0 ×
= 100.65 = 100.7%
15.6
15.5
.
.
Therefore, the new calibration value of HGB needs to be set at 100.7%. This means
that the calibration value increased 0.7%.
New calibration
value
Previous
calibration value
Average of values gained
by reference method
Average of values gained
by this instrument