Tecnai F20 - Page 18

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CSBCryoEMFacilityTF20OperatingInstructionsSEC
05/02/16
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VII. EndofSession
14. Closecolumnvalves
15. Retractthecampera
16. Putthescreendown
17. Home the stage (go to the Stage tab  Control and click on Holder, this ensures that the stage                  
isat0uminX,YandZ.
18. Removetheholdercarefully.
19. Clickoncryocycle.Makesurethecryocyclewillrunfor365minutesormore.
Wait~5minutesorforTurbototurnyellowbeforeremovingtheliquidnitrogendewar
20. Remove the dewar from the microscope and dispose of the remaining LN2 in the foam              
containerunderthetable.
21. Placetheprotectivefabricunderneaththeanticontaminator.
22. Dismountthespecimenfromtheholderanddisposeofit(orsaveit)properly.
23. IfusingtheRTorRTtomographyholders,returnthemtotheirproperreceptacle.
24. If using the Gatan cryo holder(s), return to the dry pumping station and follow the instructions               
thereonhowtowarmitandpumpitproperly.
25. CloseDigitalMicrographandtheTecnaiUserInterfaceandlogout.
26. Pleasesignupontheuser'ssheet.Recordtheactualtimespentimaging.
27. Return the next day to retrieve Data. Data will be moved overnight to the computer located               
behindthecurtainintheF20room
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