Thermo Scientific Evolution 300 - Oligo Calculator

Use the Oligo calculator:
The oligonucleotide calculator determines the following data for a base
sequence that you enter:
• Number of bases.
• Percent GC content.
• Molecular weight.
• Absorptivity (extinction coefficient).
• Conversion factor to be used in oligonucleotide measurements.
• T
m
for oligos up to 20-mers, DNA, DNA hybrids and RNA hybrids.
1. Highlight Biological Tests on the Main Menu page.
The Biological Tests sub-menu will open.
2. Choose Oligo Calculator and press Enter.
64 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific