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Thermo Scientific Evolution 300 User Manual

Thermo Scientific Evolution 300
86 pages
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Evolution™ 300 and Evolution 600
User Guide
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Thermo Scientific Evolution 300 Specifications

General IconGeneral
BrandThermo Scientific
ModelEvolution 300
CategoryMeasuring Instruments
LanguageEnglish

Summary

Introduction

Conventions used in this manual

Explains safety precautions and important information formats used in the manual.

Questions or concerns

Provides contact information for technical assistance or questions regarding the instrument.

Spectrophotometer Basics

Spectrophotometer components

Identifies major visible components of a typical Evolution spectrophotometer.

Connectors

Shows the locations and functions of various connectors on the spectrophotometer.

Accessories

Manual accessories

Describes standard rectangular cell holders and their installation process.

Installation

Details the procedure for installing and removing accessories in the sample compartment.

Standard rectangular cell holder

Describes the standard single cell holders supplied with the instrument.

Adjustable micro cell holder

Recommended for small aperture cells, allowing precise cell positioning.

Long pathlength rectangular cell holder

Accommodates rectangular cells with pathlengths from 1 to 100 mm.

Cylindrical cell holder

Designed for cylindrical cells with a 22 mm external diameter and up to 50 mm pathlength.

Thermostatted single cell holder

Accommodates square cells and operates at controlled temperatures (4-80°C).

Test tube/vial holder

Designed for test tubes or vials from 10 to 21 mm diameter and 40 to 100 mm high.

Rectangular cell holder

Allows use of 15 mm beam height filters and flow cells in the instrument.

Fiber optic sampling accessory

Enables samples to be measured outside the main sample compartment.

DRA-EV-600 Diffuse Reflectance accessory

Allows measurement of solid samples horizontally outside the instrument plane.

Solid Sample Holder

Provides capacity to measure various solid samples in % Transmittance mode.

Smart Accessories

Features auto-recognition, alignment, and serial number reporting for efficient operation.

System accessories

Discusses accessories like internal printers that are factory installed or installed by service engineers.

Installing Smart Accessories

Provides detailed steps for installing smart accessories with automatic alignment.

Using the Instrument

Local Control

Explains how function keys operate within each screen and are shown at the display bottom.

Main Menu page

Describes how to access major software functions from the main menu.

Text entry

Details how to enter text items using the text entry screen and navigation.

Editing numeric fields

Guides users on highlighting and editing numeric fields using the keypad.

Pop-up menus

Explains how to navigate and confirm choices within pop-up menus using arrow keys.

File operation

Describes how to store, view, access, and maintain files in the instrument Library or on disk.

Scan application

Provides step-by-step instructions for running a Scan application.

Fixed application

Outlines the procedure for setting up and running a Fixed application.

Quantitation application

Details the steps for setting up and running a Quantitation application.

Kinetics application

Guides users through setting up and running a Kinetics application.

Multicomponent application

Explains how to set up and run a Multicomponent Analysis (MCA) application.

Biological tests

Nucleic Acid Tests

Details how to select and run specific nucleic acid tests like DNA and RNA analysis.

Protein Tests

Covers various protein assays such as Bradford, Lowry, and BCA methods.

Cell Growth

Describes how to measure cell growth using absorbance at 600nm.

Oligo Calculator

Explains the calculator for determining oligonucleotide properties like Tm and MW.

VISIONsecurity and VISIONpro

Maintenance

Routine maintenance

Covers basic user-performed maintenance like keeping the interior dust-free.

Cleaning instrument exterior

Provides instructions for safely cleaning the exterior of the instrument.

Internal printer

Details how to open the housing and feed paper for the internal printer.

Light sources (Evolution 600 only)

Advises on handling hot lamps and cooling the instrument before removal.

Deuterium lamp

Step-by-step guide for replacing the deuterium lamp, including safety precautions.

Tungsten-halogen lamp

Step-by-step guide for replacing the tungsten-halogen lamp, including safety precautions.

Mercury lamp (Evolution 300 and Evolution 600)

Refers to a separate guide for instructions on replacing the mercury lamp.

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