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Thermo Scientific Ion 520 User Manual
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F
or Research Use Only
. Not for use in diagnostic pr
ocedures.
Ion 520
™
& Ion 530
™
ExT Kit –
Chef
USER GUIDE
for use with:
Ion Chef
™
System
Ion S5
™
System
Ion S5
™
XL System
Ion GeneStudio
™
S5 System
Ion GeneStudio
™
S5 Plus System
Ion GeneStudio
™
S5 P
rime System
Catalog Number
A30670
Publication Number
MAN0015805
Revision
F
.0
2
Table of Contents
default chapter
3
Table of Contents
3
CHAPTER 1 Product Information
6
Product Description
6
Library Compatibility
6
Ion Genestudio
6
Software Compatibility
7
Kit Contents and Storage
7
Kit Summary
7
Kit Components
7
Related Products
8
Compatible Ion Chip ™ Kits
8
Ion S5 ™ Ext Calibration Standard
8
Ion S5 ™ Controls Kit Plus
9
Coli DH10B Control 600 Library
9
Required Materials Not Supplied
9
About the Ion Chef ™ System
10
Ion Chef ™ Instrument Components
10
Interior Hardware and Consumables
11
About the Ion Chef ™ System Touchscreen Interface
12
Workflow
13
CHAPTER 2 Before You Begin
14
Precautions
14
Avoid Nucleic Acid Contamination
14
Avoid Chip Damage
14
Protection by Equipment
14
Guidelines for Using Ion Chef ™ Consumables and Reagents
15
Prepare the Ion Chef ™ Instrument for Use
15
CHAPTER 3 Create a Planned Run
17
About Planned Runs
17
Create a Planned Run
18
Planned Run Workflow Key Fields
22
Start the Ion Chef
24
Materials Required
24
Dilute the Library
24
Guidelines for Library Dilution
25
Dilute the Sample Library
25
Dilute the Control Library
25
Prepare the Consumables
25
Add the Diluted Libraries to the Ion Chef
26
Library Sample Tube
26
System
27
Load the Ion Chef
27
Load the Tip Racks and PCR Plate
28
Load the Reagents and Solutions Cartridges
29
Load the Recovery Tubes and Enrichment Cartridge V2
31
Load the Chip-Loading Centrifuge
33
Confirm that Consumables Are Correctly Installed
35
Pre-Run Checklist
36
Run
37
Start the Ion Chef
37
Unload the Chip for Sequencing
42
Initialize the Sequencer
45
Ion Genestudio ™ S5 Systems Component Positions
45
Before You Begin
46
When a Manual Cleaning of the Sequencer Is Required
46
Initialize the Sequencer
47
CHAPTER 6 Start the Sequencing Run
49
Chip Handling Guidelines
49
Start the Sequencing Run
49
Maintain the Sequencer
52
Materials Required
52
Clean or Decontaminate the Sequencer
52
Reagent Consumables Disposal
52
Scrubber Removal and Disposal
52
Recycle Ion S5
53
Ext Wash Solution and Ion S5
53
Cleaning Solution Bottles
53
About the Cleaning Protocol
54
Materials Required
54
Instrument
54
System
54
Remove and Dispose of Used Consumables
55
Inspect and Clean the Recovery Centrifuges and Buckets
57
Start the Cleaning
58
APPENDIX A Troubleshooting
60
Ion Chef ™ System Setup and Operation
60
Ion S5 /Ion Genestudio ™ S5 Systems Setup and Operation
63
Instrument Alarms and Events
63
Initialization-General Errors
64
APPENDIX B Supplemental Procedures
65
Perform a Manual Cleaning of the Sequencer
65
Perform an Instrument Reset Run with an Initialized Sequencer that Is Loaded with an Unused Reagents Cartridge
66
Quality Control of Ion Sphere ™ Particles
67
Quality Control Using the Guava
67
Maintain the Ion Chef ™ System
67
APPENDIX C Safety
68
Symbols on this Instrument
68
Conformity Symbols
69
Location of Safety Labels on this Instrument
70
Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
70
Instrument Safety
70
General
70
Physical Injury
70
Electrical Safety
71
Cleaning and Decontamination
71
Ultraviolet (UV) Safety
71
Safety and Electromagnetic Compatibility (EMC) Standards
71
Safety Compliance
72
Emc
72
Environmental Design
72
Instrument Safety - Ion S5
73
Ion Genestudio ™ S5 Systems
73
Ion Genestudio S5 Systems
73
Chemical Safety
73
Biological Hazard Safety
74
Documentation and Support
75
Related Documentation
75
Customer and Technical Support
76
Limited Product Warranty
76
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Thermo Scientific Ion 520 Specifications
General
Brand
Thermo Scientific
Model
Ion 520
Category
Laboratory Equipment
Language
English
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