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Thermo Scientific ISE User Manual

Thermo Scientific ISE
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User Guide
Fluoride Ion
Selective
Electrode
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Thermo Scientific ISE Specifications

General IconGeneral
CategoryMeasuring Instruments
ManufacturerThermo Scientific
Measurement RangeDependent on electrode type
ResolutionDependent on electrode type
AccuracyDependent on electrode type
Electrode TypesISE (Ion Selective Electrodes)
DisplayLCD
Power SupplyAC adapter or battery
DimensionsVaries by model
WeightVaries by model

Summary

Introduction

Fluoride ionplus® Sure-Flow® Solid State Combination ISE

Description of the combination fluoride electrode.

Fluoride Solid State Half-Cell ISE

Description of the fluoride half-cell electrode.

Required Equipment

Fluoride calibration standards.

Catalog numbers and descriptions of fluoride calibration standards.

Fluoride electrode filling solution.

Details on filling solutions for different electrode types.

Total Ionic Strength Adjustment Buffer (TISAB)

Low-Level TISAB

Preparation of TISAB for low-level fluoride measurements.

TISAB IV

Preparation of TISAB IV for complex samples.

Electrode Setup

Electrode Preparation

Preparation steps for half-cell and combination fluoride electrodes.

Electrode Filling Instructions

Step-by-step guide for filling the electrode with solution.

Checking Electrode Operation (Slope)

Measurement Units

Sample Requirements

Details on sample temperature, composition, and TISAB usage.

Measuring Hints

Electrode Storage

Electrode Maintenance

Polishing the Sensing Surface of Fluoride Electrodes

Procedure to restore the sensing surface using toothpaste.

Flushing Clogged Electrodes

Procedure to flush the electrode junction if clogged.

Disassembling and Reassembling Electrodes

Reassembling Electrodes

Instructions for reassembling the electrode after maintenance.

Analytical Techniques

Direct Calibration

Simple procedure for measuring multiple samples with standards.

Incremental Techniques

Methods for measuring samples without calibration curves.

Known Addition

Technique for measuring dilute samples or in complexing agents.

Known Subtraction

Quick titration method for species with no stable standards.

Analate Addition

Method for viscous or concentrated samples.

Analate Subtraction

Measurement of ions where no ISE exists.

Titrations

Using electrode as endpoint detector for fluoride titration.

Typical Calibration Curve

Direct Calibration

Direct Calibration Setup

Preparing the electrode and meter for direct calibration.

Serial Dilutions for Standards

Method for preparing standards using serial dilution.

Fluoride Standards with TISAB II

Direct Calibration with ISE Mode

Direct Calibration with Millivolt Mode

Low-Level Measurements

Low-Level Setup

Setting up the electrode and meter for low-level measurements.

Low-Level Calibration with Autoblank

Calibration using an ISE meter with Autoblank.

Low-Level Calibration with Millivolt Mode

Single Known Addition

Single Known Addition Setup

Guideline For Known Addition

Table providing guidelines for known addition volumes and concentrations.

Known Addition Using Meter's Known Addition Mode

Known Addition Using Meter's Millivolt Mode

Titrations

Fluoride in Acid Solutions

Procedure for measuring fluoride in acidic samples.

Fluoride in Alkaline Solutions

Electrode Characteristics

Electrode Response

Describes electrode potential response and time.

Reproducibility

Factors affecting electrode measurement reproducibility.

Limits of Detection

Minimum detectable fluoride concentration.

Temperature Effects

How temperature affects electrode potential and slope.

Interferences

Anions and cations that may interfere with measurements.

pH Effects

Impact of pH on fluoride measurements and interferences.

Complexation

How complexing agents affect fluoride measurements.

Theory of Operation

Explanation of how the fluoride electrode works.

Reference Electrode Conditions

Troubleshooting

Meter Troubleshooting

Troubleshooting steps for the measurement meter.

Electrode Troubleshooting

Troubleshooting steps for the electrode.

Sample/Application Considerations

Technique Review

Reviewing procedures and ensuring proper technique.

Assistance and Support

Contact information for technical support.

Warranty Information

Information on product warranty.

Troubleshooting Checklist

Ordering Information

Specifications

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