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Thermo Scientific LTQ Series User Manual
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For Research Use Only
Not for use in Diagnostic Procedures
LT
Q
X
L
®
Getting Started
97355-97042
Revision A
June 2006
2
Table of Contents
Default Chapter
7
Table of Contents
7
Preface
11
About this Guide
11
Related Documentation
11
Safety and Special Notices
11
Contacting Us
12
Assistance
12
Changes to the Manual and Online Help
12
Chapter 1 Introduction
13
Introduction
13
Why Use the LTQ XL MS Detector
14
Which MS Detector Technique-ESI or APCI-Is Better for Analyzing My Samples
16
Using ESI/MS
16
Using APCI/MS
17
Should I Use Sheath, Auxiliary, And/Or Sweep Gases
19
How Can I Introduce My Samples into the MS Detector
20
What Types of Buffers Should I Use? What Types Should I Avoid
22
How Should I Set up the MS Detector for Various LC Flow Rates
23
What Is Tuning and Calibration of the MS Detector All about
25
What Is Tuning and Calibration of the MS Detector All about
26
What Is Tuning and Calibration of the MS Detector All about
27
What Types of Experiments Can I Perform with the LTQ XL MS Detector
28
General MS or Msn Experiments
28
Data-Dependent Experiments
29
Ion Mapping Experiments
32
Ion Tree Experiments
35
Chapter 2 Setting up the Ion Source for Tuning and Calibrating the MS
40
Placing the LC/MS System in Standby
40
Removing the APCI Probe
41
Removing the Ion Max Ion Source Housing
45
Installing the Ion Sweep Cone
46
Installing the Ion Max Ion Source Housing
48
Installing the ESI Probe
51
Chapter 3 Tuning and Calibrating Automatically in the ESI/MS Mode
55
Setting up the Syringe Pump for Tuning and Calibration
57
Setting up the MS Detector in the Xcalibur Data System for Tuning and Calibration
59
Tuning the MS Detector Automatically in the ESI/MS Mode
68
Saving Your ESI/MS Tune Method
72
Calibrating the MS Detector Automatically
74
Cleaning the MS Detector after Tuning and Calibrating
77
Tuning with Your Analyte in LC/ESI/MS Mode
81
Chapter 4 Tuning with Your Analyte in LC/ESI/MS Mode
82
Setting up to Introduce Sample by Syringe Pump into Solvent Flow from an LC
83
Setting up to Tune the MS Detector with Your Analyte
86
Optimizing the MS Detector Tune Automatically with Your Analyte
89
Saving the ESI/MS Tune Method
93
Acquiring ESI Sample Data Using the Tune Plus Window
95
Setting up to Acquire MS/MS Data in the Full Scan Type
96
Optimizing the Isolation Width and Setting up to Optimize the Collision Energy
96
Chapter 5 Acquiring ESI Sample Data Using the Tune Plus Window
96
Optimizing the Collision Energy Automatically for an MS/MS Experiment
101
Setting up to Introduce Sample by Loop Injection into Solvent Flow from an LC
104
Acquiring MS Data in the SIM Scan
106
Chapter 6 Setting up the Ion Source for Acquiring Data in APCI/MS/MS
114
Removing the ESI Probe
114
Removing the Ion Max Ion Source Housing
117
Removing the Ion Sweep Cone
118
Installing the Corona Needle
119
Installing the Ion Max Ion Source Housing
120
Installing the APCI Probe
123
Chapter 7 Optimizing the MS Detector with Your Analyte in APCI/MS
127
Mode115
127
Setting up the Inlet for Tuning Using High-Flow Infusion
128
Setting up the MS Detector for APCI/MS Operation
131
Optimizing the Tune of the MS Detector Automatically in APCI/MS Mode
135
Saving the APCI/MS Tune Method
138
Cleaning the MS Detector after Tuning in APCI Mode
140
Acquiring APCI Sample Data Using the Tune Plus Window
143
Setting up to Introduce Sample by Loop Injection into Solvent Flow from an LC
144
Chapter 8 Acquiring APCI Sample Data Using the Tune Plus Window
144
Acquiring APCI Data in the SIM Scan Mode
146
Appendix A Sample Formulations
151
Caffeine, MRFA, and Ultramark 1621 Stock Solutions
152
Stock Solution: Caffeine
153
Stock Solution: MRFA
154
Stock Solution: Ultramark 1621
154
ESI Calibration Solution: Caffeine, MRFA, Ultramark 1621
155
Stock Solution: Reserpine
156
Reserpine Tuning Solution and Reserpine APCI Sample Solution
156
Appendix Bltq XL High Mass Range Calibration
159
High Mass Range Calibration Solution
160
Normal Mass Range Calibration
161
Enter Normal Mass Range Data into Tune Plus
162
Tune on M/Z 524.3
164
High Mass Range Calibration Procedure
166
Notes
171
Index
173
Other manuals for Thermo Scientific LTQ Series
Hardware Manual
174 pages
Getting Started Guide
74 pages
4
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Thermo Scientific LTQ Series Specifications
General
Brand
Thermo Scientific
Model
LTQ Series
Category
Laboratory Equipment
Language
English
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