Thermo Scientific Orbitrap ID-X User Manual

Orbitrap Tribrid Series
Getting Started Guide
80000-97026 Revision A June 2018

Thermo Scientific Orbitrap ID-X Specifications

General

Scan Rate	Up to 40 Hz
Mass Analyzer	Orbitrap
Mass Accuracy	2 ppm
Ion Source Options	APCI, APPI
MSn	up to MSn
Software	Thermo Scientific Xcalibur

Summary

Regulatory Compliance

Low Voltage Directive 2014/35/EU

EMC Directive 2014/30/EU & EMC Standards

Orbitrap Fusion and Orbitrap ID-X Compliance Details

Proper Use of Thermo Scientific Instruments

Guidelines for proper instrument use ensuring safety and performance.

Introduction

Ionization Techniques

Setting Up the Ion Source

Preparing the Mass Spectrometer

Steps to prepare MS before ion source installation.

Installing the Ion Source

Procedure for installing ion source without tools.

Preparing the Spray Insert

Spray insert installation and positioning.

Connecting the Inlet Plumbing

PEEK Fittings and Tubing Guidelines

Setting Up the Syringe and Syringe Pump

Setting up syringe pump for sample infusion.

Setting Up Inlet Plumbing for Sample Introduction

Techniques for introducing samples into the ion source.

Preparing the System for Calibration

Setting Up Syringe Pump for Direct Infusion

Setting up syringe pump for direct infusion of calibration solution.

Setting Up Mass Spectrometer for Calibration

Configuring MS parameters for calibration.

Establishing a Stable Ionization Spray

Evaluating Spray Stability

Methods to evaluate ionization spray stability.

Optimizing Ion Source Parameters

Adjusting ion source parameters for optimal performance.

Calibrating the Mass Spectrometer

Calibration Solution Peak Values

Expected peak values for calibration solutions in positive/negative modes.

Running Calibration Procedures

Acquiring Sample Data with Tune

Setting Up LC/MS for Analyte Optimization

Defining Scan Parameters for Precursor Optimization

Acquiring Data File Using Tune Application

Acquiring Data with Xcalibur

Acquiring Data File Using Xcalibur Data System