INTENMTATION
1
1.
>
-
Interpretation oFresul&
is
2
-
>
c
chosen:
1
+
>
-:
results above cut off value
are presented with
+,
results below
cut off are presented with
-
2
-
>
+:
results above cut offv~lne
are presented with
9
results
it are presented with
6
odule
4:
Cubic Sgtline (optional)
The tbllowing measurement modes are available in module 4:
1
Absorbanees are measured at one wavelength
2
DUAL
WAmLENGm: Absorbanees are measured usmg two wavelengths.
Results of the second measurement are subtracted from results of the
first measurement.
3
TWOPBTNT:
Absorbances of the plate are measured at two separate polnts ~n
time
Results of the first measurement are subtracted from the results ofthe
second measumnent.
4
KINETIC:
Rates of absorbance change
are
measured
The following measurement parameters can be selected:
FILER
In
measurement modes
1,
2,
3
and
4.
Enter the wavelmgth(s) of the
filter($ or the filter number(@ (see
7
FILER
3.6
General parameters).
The following blanking possibilities are available in modes
1,
2,
3
and 4:
NO
REAGENT BLA
SINGLE
WELL
REAGENT BLANK
-blank well is entered
COL-W
MEAN
BLA
-blank column (1-12)
is
entered
-
average
of
the blank values
(8)
of the column
is
used
COL BE
-blank column (1-12)
is
entered
-
each row will have an individual blank value which is used
ROW MEAN BLA
-blank row (A-W) is entered
-
average of the blank values
(12)
of the row
is
used
ROW BLANK
-blank row (A-I-f)
is
entered
-
each column will have an individual bla
value which is used
1
Ern
2
ALL
Blanking on every plate
(I
BL
ON
EVERY
PL)
or retained
blank
(2
RETAImD
BL
,
blank value
of
a former plate
i
also be selected.
If
ed blank
is
selected,
1
USE
OLD
NEW BLANK NOW will be asked before reading the next plate.
In nrodes
2,
3
and
4
final results
(e.g.
absorbance differc~~ees) or also
intermediate results (e.g. absorbances with filter
I
and filter
2)
can
selected.
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