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ThermoFisher Scientific QuantStudio 3 User Manual

ThermoFisher Scientific QuantStudio 3
66 pages
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For Research Use Only. Not for use in diagnostic procedures.
QuantStudio
Design and Analysis v2
USER GUIDE
for use with:
QuantStudio
7 Pro Real-Time PCR System
QuantStudio
6 Pro Real-Time PCR System
QuantStudio
5 Real-Time PCR System
QuantStudio
3 Real-Time PCR System
QuantStudio
1 Real-Time PCR System
Publication Number MAN0018202
Revision F.0

Table of Contents

Other manuals for ThermoFisher Scientific QuantStudio 3

Questions and Answers:

ThermoFisher Scientific QuantStudio 3 Specifications

General IconGeneral
BrandThermoFisher Scientific
ModelQuantStudio 3
CategoryLaboratory Equipment
LanguageEnglish

Summary

About the software

Compatible instruments

Lists the QuantStudio real-time PCR systems compatible with the software.

Compatible data files

Lists the real-time PCR instruments whose data files are compatible with the software.

Workflow

Set up a plate file

Select a system template or existing plate file to set up a new plate file

Guide on creating a new plate file by selecting a system template or existing file.

Confirm or edit run method

Details on how to review and modify the run method parameters for an experiment.

Confirm or edit plate setup

Procedures for setting up the plate layout, including selecting wells and assigning samples.

Set up the standard curve

Detailed steps for configuring standard curves for quantitative analysis.

Review and analyze data

Workflow: General procedures to review the primary analysis results

Overview of the steps involved in reviewing and analyzing the data generated from an instrument run.

Open a data file

Instructions for opening existing data files for analysis and review.

Review results in the Amplification Plot

Steps to examine amplification curves, baseline, and threshold settings in the Amplification Plot.

Identify and omit outliers from analysis

How to identify wells with outlier Cq values and exclude them from analysis.

Edit primary analysis settings

Procedures for adjusting primary analysis parameters like Cq, Melt, and QC settings.

Override the calibration data

Steps to replace instrument calibration data with data from another instrument.

Export results

Export the Well Table

How to export the well data, including Cq values and status, to a file.

Generate a report

Steps to create a customizable report summarizing experiment results.

General procedures to analyze data in the Quality Check tab

View the post-run summary

How to access and review a summary of the instrument run details.

Review individual well results in the Well Table

Procedures for examining detailed results for each well in the Well Table.

Manage plate files and data files

Save a plate file or data file

Instructions for saving plate files and data files, including options for new names.

Manage analysis modules

About analysis modules

Explanation of analysis modules as plugins for extending software functionality.

Select an analysis module

How to choose and activate an analysis module for specific data analysis tasks.

Manage export settings

View or edit export settings

Instructions for customizing which data columns and types are included in exports.

About data analysis

About the quantification cycle (Cq)

Explanation of the quantification cycle (Cq) and its role in gene expression analysis.

Guidelines for manual threshold and baseline settings for Cq analysis

Recommendations for manually setting thresholds and baselines for accurate Cq determination.

Troubleshooting

Documentation and support

Information on accessing user guides, technical support, and other resources.

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