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ThermoFisher Scientific QuantStudio 12K Flex OpenArray AccuFill System User Manual

ThermoFisher Scientific QuantStudio 12K Flex OpenArray AccuFill System
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For Research Use Only. Not for use in diagnostic procedures.
QuantStudio
12K Flex OpenArray
AccuFill
System
USER GUIDE
Use and maintenance
for use with:
OpenArray
AccuFill
Instrument
OpenArray
AccuFill
Software v2.0
Catalog Number 4471021
Publication Number MAN0025669
Revision A.0

Table of Contents

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ThermoFisher Scientific QuantStudio 12K Flex OpenArray AccuFill System Specifications

General IconGeneral
BrandThermoFisher Scientific
ModelQuantStudio 12K Flex OpenArray AccuFill System
CategoryLaboratory Equipment
LanguageEnglish

Summary

Chapter 1 Product Information

Product Description

Provides an overview of the QuantStudio™ 12K Flex OpenArray™ AccuFill™ System's capabilities and components.

About the Instrument

Details the QuantStudio™ 12K Flex OpenArray™ AccuFill™ Instrument's functionality and design.

About the Software

Explains the features and requirements of the OpenArray™ AccuFill™ Software v2.0.

Workflow

Outlines the sequence of operations for the OpenArray™ AccuFill™ Software v2.0.

Chapter 2 Basic Operations and One-Time Procedures

Basic Instrument Operations

Covers fundamental actions required to operate the QuantStudio™ 12K Flex instrument.

Important Guidelines for Handling an OpenArray™ Plate

Provides crucial instructions for safely and correctly handling OpenArray™ Plates.

Set the Preferences

Guides users on configuring software settings for instrument operation and data management.

Chapter 3 Initialize the System

Start the System

Details the initial steps to power on and begin operating the instrument system.

Set Up the System

Explains the procedure for preparing the instrument deck for operation.

Perform a System Self-Test

Describes how to run diagnostic tests to verify instrument functionality.

Overview of the Deck Screen

Introduces the main user interface of the instrument control software.

Chapter 4 Download or Prepare TPF or SPF Files

Download TPF or SPF Files

Instructions for obtaining TPF or SPF files required for run setup.

Create TPF or SPF Files from a Template

Guidance on generating TPF or SPF files using predefined templates.

Chapter 5 Map Plates

Overview of Map Plates Features

Introduces the functionality and purpose of the Map Plates feature.

Configure the Experiment Design to Map the Plates

Steps to define sample mapping configurations for OpenArray™ Plates.

Map a 384-Well Plate from 96-Well Plates

Details on mapping samples from 96-well plates to a 384-well OpenArray™ Plate.

Export Plate Files

Instructions on exporting mapped plate data in various file formats.

Chapter 6 Set Up and Start the Run with the Quick Run Workflow

Overview of the Quick Run Workflow

Introduces the simplified workflow for running experiments quickly.

Before You Begin—Quick Run Workflow

Preparation steps and requirements before starting a quick run.

Configure the Run—Quick Run Workflow

Steps to set up experiment parameters for a quick run.

Verify the Run Setup and Start the Run—Quick Run Workflow

Final checks and initiation of a quick run experiment.

Chapter 7 Set Up and Start a Run with the Full Run Workflow

Overview of the Full Run Workflow

Introduces the comprehensive workflow for detailed experiment setup.

Before You Begin—Full Run Workflow

Preparation steps and requirements before starting a full run.

Configure the Experiment Design for the Full Run Workflow

Steps to define experiment parameters for a full run.

Verify the Run Setup and Start the Run—Full Run Workflow

Final checks and initiation of a full run experiment.

Chapter 8 Monitor Run Progress

Deck Screen During a Run

Visual overview of the instrument's status and components during an active run.

Cancel a Run

Procedure for stopping an ongoing experiment run on the instrument.

Chapter 9 Prepare the OpenArray™ Plate for PCR

Remove the OpenArray™ Plate from the Instrument

Instructions for safely detaching a filled OpenArray™ Plate.

Seal the OpenArray™ Plate

Steps for sealing the OpenArray™ Plate with its case and lid.

Appendix A Cleaning and Maintenance of the OpenArray™ AccuFill™ Instrument

Cleaning Schedule and Instructions

Guidelines and procedures for routine cleaning of the instrument.

Spill Accidents

Procedures to follow in case of accidental spills inside the instrument.

Appendix B Required Materials Not Supplied

Appendix C Site Requirements

Dimensions and Weights

Provides physical specifications for instrument installation and transport.

Environmental Requirements

Specifies the necessary environmental conditions for instrument operation.

Electrical Requirements

Details power supply and connection requirements for safe instrument operation.

Computer Requirements

Lists the minimum specifications for the computer running the instrument software.

Appendix D Manage the Service Settings

View the Instrument Statistics

Accessing and viewing performance data and lifetime metrics of the instrument.

View the Status of the Sensors

Checking the operational status of various sensors within the instrument.

Perform Diagnostics Tests

Guides on running various tests to diagnose instrument issues.

View Loading History Log

Accessing records of sample loading operations performed by the instrument.

Appendix E Safety

Symbols on this Instrument

Explains hazard and attention symbols found on the instrument.

Instrument Safety

General safety precautions and warnings related to operating the instrument.

Chemical Safety

Guidelines for safe handling, storage, and disposal of chemicals.

Appendix F Documentation and Support

Customer and Technical Support

Information on how to obtain assistance and support for the product.

Limited Product Warranty

Details the terms and conditions of the product's warranty.

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