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ThermoFisher Scientific QuantStudio 5 User Manual

ThermoFisher Scientific QuantStudio 5
66 pages
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For Research Use Only. Not for use in diagnostic procedures.
QuantStudio
Design and Analysis v2
USER GUIDE
for use with:
QuantStudio
7 Pro Real-Time PCR System
QuantStudio
6 Pro Real-Time PCR System
QuantStudio
5 Real-Time PCR System
QuantStudio
3 Real-Time PCR System
QuantStudio
1 Real-Time PCR System
Publication Number MAN0018202
Revision F.0

Table of Contents

Other manuals for ThermoFisher Scientific QuantStudio 5

Questions and Answers:

ThermoFisher Scientific QuantStudio 5 Specifications

General IconGeneral
TypeReal-Time PCR System
Number of Channels5
Detection MethodFluorescence
ConnectivityUSB, Ethernet
Excitation SourcesLED
Temperature Accuracy±0.25°C
Frequency50/60 Hz
Sample Capacity96 samples
Temperature Range4°C to 99.9°C
SoftwareQuantStudio Design and Analysis Software
Supported ApplicationsGene Expression, Genotyping, Copy Number Variation
Run TimeVariable, depending on protocol
Voltage100–240 VAC

Summary

About the Software

Set Up a Plate File

Select System Template or Plate File

Guides users on selecting system templates or existing plate files for new plate file creation.

Confirm or Edit Run Method

Details on modifying run parameters like temperature, time, ramp rate, and stages.

Confirm or Edit Plate Setup

Steps for configuring plate layout, selecting wells, and assigning samples or targets.

Manage Samples and Targets

Procedures for adding, assigning, and managing sample and target information in plate setup.

Configure Reagents and Standards

Setting up reagents, selecting passive references, and configuring standard curves for analysis.

Review and Analyze Data

Open a Data File

Instructions for importing and opening data files for analysis within the software.

Review Amplification Plot

Guidance on evaluating amplification curves, settings, and status in the Amplification Plot.

Review QC Alerts and Edit Analysis Settings

Checking Quality Control alerts and adjusting core analysis parameters like Cq and Melt settings.

General Procedures for Analyzing Data in the Quality Check Tab

Manage Plate Files and Data Files

Manage Export Settings

About Data Analysis

Primary Analysis Settings and Guidelines

Overview of primary analysis settings and guidelines for manual threshold/baseline adjustments.

Appendix A Troubleshooting

Troubleshooting: Fluorescence & Communication Issues

Resolving common issues like high fluorescence signals and instrument communication problems.

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